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. 1993 Nov;46(11):1024–1029. doi: 10.1136/jcp.46.11.1024

Malignant transformation of osteoblastoma: study using image analysis microdensitometry.

J Grace 1, S McCarthy 1, R Stankovic 1, W Marsden 1
PMCID: PMC501687  PMID: 8254089

Abstract

AIM--To determine if the malignant transformation, as perceived histologically, in a case of osteoblastoma from the right femur, was also expressed as a quantitative change in nuclear DNA during tumour progression over five months. METHODS--Nuclear DNA microdensitometry by computer image analysis was used to acquire relative DNA distribution patterns. Tissue had been removed on four separate occasions from a lesion in the right femur of an 18 year old man. Retrospective DNA analysis was performed on formalin fixed, paraffin wax-embedded tissue. RESULTS--The DNA profile of the initial biopsy specimen, which was histologically diagnosed as osteoblastoma, was euploid with a near diploid (2c) modal DNA. The second biopsy specimen taken one month later also resembled osteoblastoma but showed an aneuploid DNA profile with a diploid modal DNA and some nuclei with ploidy greater than 5c. The third biopsy specimen taken four months later showed histological evidence of osteosarcoma and a near pentaploid (5c) modal DNA with large number of nuclei exceeding 5c. CONCLUSIONS--DNA microdensitometry confirmed the initial and final diagnosis. The technique also seems to be capable of detecting aneuploidy before malignancy is morphologically evident.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkin N. B. Cytophotometric DNA determination correlated to karyotype, particularly in cancer. Anal Quant Cytol Histol. 1987 May;9(2):96–104. [PubMed] [Google Scholar]
  2. Bauer H. C., Kreicbergs A., Silfverswärd C., Tribukait B. DNA analysis in the differential diagnosis of osteosarcoma. Cancer. 1988 Apr 1;61(7):1430–1436. doi: 10.1002/1097-0142(19880401)61:7<1430::aid-cncr2820610725>3.0.co;2-m. [DOI] [PubMed] [Google Scholar]
  3. Bertoni F., Unni K. K., McLeod R. A., Dahlin D. C. Osteosarcoma resembling osteoblastoma. Cancer. 1985 Jan 15;55(2):416–426. doi: 10.1002/1097-0142(19850115)55:2<416::aid-cncr2820550221>3.0.co;2-5. [DOI] [PubMed] [Google Scholar]
  4. Böcking A., Adler C. P., Common H. H., Hilgarth M., Granzen B., Auffermann W. Algorithm for a DNA-cytophotometric diagnosis and grading of malignancy. Anal Quant Cytol. 1984 Mar;6(1):1–8. [PubMed] [Google Scholar]
  5. Böcking A., Auffermann W. Algorithm for DNA-cytophotometric diagnosis and grading of malignancy. Anal Quant Cytol Histol. 1986 Dec;8(4):363–363. [PubMed] [Google Scholar]
  6. Decosse J. J., Aiello N. Feulgen hydrolysis: effect of acid and temperature. J Histochem Cytochem. 1966 Aug;14(8):601–604. doi: 10.1177/14.8.601. [DOI] [PubMed] [Google Scholar]
  7. Eneroth C. M., Zetterberg A. Microspectrophotometric DNA analysis of malignant salivary gland tumours. Acta Otolaryngol. 1974 Apr;77(4):289–294. doi: 10.3109/00016487409124627. [DOI] [PubMed] [Google Scholar]
  8. Friedlander M. L., Hedley D. W., Taylor I. W. Clinical and biological significance of aneuploidy in human tumours. J Clin Pathol. 1984 Sep;37(9):961–974. doi: 10.1136/jcp.37.9.961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gimenez I. B., Conti C. J., Cabrini R. L. The effect of decalcification on the microspectrophotometric determination of DNA. Stain Technol. 1977 Nov;52(6):352–353. doi: 10.3109/10520297709116813. [DOI] [PubMed] [Google Scholar]
  10. Heliö H., Karaharju E., Nordling S. Flow cytometric determination of DNA content in malignant and benign bone tumours. Cytometry. 1985 Mar;6(2):165–171. doi: 10.1002/cyto.990060213. [DOI] [PubMed] [Google Scholar]
  11. Jackson R. P. Recurrent osteoblastoma: a review. Clin Orthop Relat Res. 1978 Mar-Apr;(131):229–233. [PubMed] [Google Scholar]
  12. Jarvis L. R. A microcomputer system for video image analysis and diagnostic microdensitometry. Anal Quant Cytol Histol. 1986 Sep;8(3):201–209. [PubMed] [Google Scholar]
  13. Kreicbergs A., Broström L. A., Cewrien G., Einhorn S. Cellular DNA content in human osteosarcoma: aspects on diagnosis and prognosis. Cancer. 1982 Dec 1;50(11):2476–2481. doi: 10.1002/1097-0142(19821201)50:11<2476::aid-cncr2820501136>3.0.co;2-n. [DOI] [PubMed] [Google Scholar]
  14. Kreicbergs A., Silvferswärd C., Tribukait B. Flow DNA analysis of primary bone tumors. Relationship between cellular DNA content and histopathologic classification. Cancer. 1984 Jan 1;53(1):129–136. doi: 10.1002/1097-0142(19840101)53:1<129::aid-cncr2820530123>3.0.co;2-o. [DOI] [PubMed] [Google Scholar]
  15. Kreicbergs A., Zetterberg A. Cytophotometric DNA measurements of chondrosarcoma: methodologic aspects of measurements in tissue sections from old paraffin-embedded specimens. Anal Quant Cytol. 1980 Jun;2(2):84–92. [PubMed] [Google Scholar]
  16. Kreicbergs A., Zetterberg A., Söderberg G. The prognostic significance of nuclear DNA content in chondrosarcoma. Anal Quant Cytol. 1980 Dec;2(4):272–279. [PubMed] [Google Scholar]
  17. Lockart R. Z., Pezzella K. M., Kelley M. M., Toy S. T. Features independent of stain intensity for evaluating Feulgen-stained cells. Anal Quant Cytol. 1984 Jun;6(2):105–111. [PubMed] [Google Scholar]
  18. Mankin H. J., Gebhardt M. C., Springfield D. S., Litwak G. J., Kusazaki K., Rosenberg A. E. Flow cytometric studies of human osteosarcoma. Clin Orthop Relat Res. 1991 Sep;(270):169–180. [PubMed] [Google Scholar]
  19. Merryweather R., Middlemiss J. H., Sanerkin N. G. Malignant transformation of osteoblastoma. J Bone Joint Surg Br. 1980 Aug;62(3):381–384. doi: 10.1302/0301-620X.62B3.6931831. [DOI] [PubMed] [Google Scholar]
  20. Miller D. M., Blume S., Borst M., Gee J., Polansky D., Ray R., Rodu B., Shrestha K., Snyder R., Thomas S. Oncogenes, malignant transformation, and modern medicine. Am J Med Sci. 1990 Jul;300(1):59–69. doi: 10.1097/00000441-199007000-00013. [DOI] [PubMed] [Google Scholar]
  21. Mirra J. M., Kendrick R. A., Kendrick R. E. Pseudomalignant osteoblastoma versus arrested osteosarcoma: a case report. Cancer. 1976 Apr;37(4):2005–2014. doi: 10.1002/1097-0142(197604)37:4<2005::aid-cncr2820370452>3.0.co;2-0. [DOI] [PubMed] [Google Scholar]
  22. Näslund I., Rubio C. A., Auer G. U. Nuclear DNA changes during pathogenesis of squamous carcinoma of the cervix in 3,4-benzopyrene-treated mice. Anal Quant Cytol Histol. 1987 Oct;9(5):411–418. [PubMed] [Google Scholar]
  23. Pieterse A. S., Vernon-Roberts B., Paterson D. C., Cornish B. L., Lewis P. R. Osteoid osteoma transforming to aggressive (low grade malignant) osteoblastoma: a case report and literature review. Histopathology. 1983 Sep;7(5):789–800. doi: 10.1111/j.1365-2559.1983.tb02291.x. [DOI] [PubMed] [Google Scholar]
  24. Revell P. A., Scholtz C. L. Aggressive osteoblastoma. J Pathol. 1979 Apr;127(4):195–198. doi: 10.1002/path.1711270406. [DOI] [PubMed] [Google Scholar]
  25. Schajowicz F., Lemos C. Malignant osteoblastoma. J Bone Joint Surg Br. 1976 May;58(2):202–211. doi: 10.1302/0301-620X.58B2.932083. [DOI] [PubMed] [Google Scholar]
  26. Seki T., Fukuda H., Ishii Y., Hanaoka H., Yatabe S. Malignant transformation of benign osteoblastoma. A case report. J Bone Joint Surg Am. 1975 Apr;57(3):424–426. [PubMed] [Google Scholar]
  27. Xiang J. H., Spanier S. S., Benson N. A., Braylan R. C. Flow cytometric analysis of DNA in bone and soft-tissue tumors using nuclear suspensions. Cancer. 1987 Jun 1;59(11):1951–1958. doi: 10.1002/1097-0142(19870601)59:11<1951::aid-cncr2820591119>3.0.co;2-x. [DOI] [PubMed] [Google Scholar]

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