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. 1993 Dec;46(12):1080–1084. doi: 10.1136/jcp.46.12.1080

New antiserum against Ki-67 antigen suitable for double immunostaining of paraffin wax sections.

G Key 1, J L Petersen 1, M H Becker 1, M Duchrow 1, C Schlüter 1, J Askaa 1, J Gerdes 1
PMCID: PMC501714  PMID: 7506714

Abstract

AIMS--To prepare a rabbit antiserum equivalent to MIB 1 to permit the simultaneous assessment of cell proliferation and other markers of interest using double labelling studies. METHODS--Rabbits were immunised with a synthetic peptide deduced from the cDNA sequence coding for the Ki-67 antigen. Serum samples were tested for immunoreactivity using different immunobiochemical methods. RESULTS--A polyclonal antiserum was derived which detects the native as well as recombinant parts of the Ki-67 antigen in different test systems. Furthermore, the antiserum stains the Ki-67 antigen in routinely processed, paraffin wax embedded material. CONCLUSIONS--After antigen unmasking by microwave treatment the antiserum described here represents a powerful tool for the determination of growth fractions even in archival material. It is especially suitable for double staining experiments in combination with monoclonal antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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