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. 1993 Dec;46(12):1109–1112. doi: 10.1136/jcp.46.12.1109

Estimation of urinary aldosterone using thin-layer chromatography and fluorimetry.

D Mattingly 1, H Martin 1, C M Tyler 1
PMCID: PMC501721  PMID: 8282834

Abstract

AIMS--To develop a fluorimetric method for the estimation of urinary aldosterone; to establish a normal range in 24 hour and overnight urine samples; and to investigate the use of overnight urines for detecting hyperaldosteronism. METHODS--Essential steps include hydrolysis of the 18 conjugate to release aldosterone and its oxidation with Benedict's solution, followed by thin-layer chromatography on silica gel and development of fluorescence on the plate with sulphuric acid. RESULTS--There was a linear correlation between the amount of aldosterone and the area under the peak on the chromatogram. The mean intra-assay and interassay coefficients of variation were 4.4% and 6.8%, respectively. The mean aldosterone excretion in 67 adults was 15.7 (SD 8.1) nmol/24 hours. The mean overnight excretion in 65 adults was 2.6 (1.4) nmol/8 hours. The method detected raised concentrations in patients with primary and secondary aldosteronism. CONCLUSIONS--This technique provides an accurate means of assaying urinary aldosterone. Overnight estimations seem to be as effective as 24 hour assays for identifying patients with hyperaldosteronism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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