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. 1993 Dec;46(12):1116–1119. doi: 10.1136/jcp.46.12.1116

Serological investigation into the association between Streptococcus bovis and colonic cancer.

R Darjee 1, A P Gibb 1
PMCID: PMC501723  PMID: 8282836

Abstract

AIMS--To determine if there was an increase in antibody titre to Streptococcus bovis in patients with colonic cancer, and if this might be a useful marker of the presence for colonic cancer. METHODS--Serum samples from 16 patients and 16 age matched controls were tested by immunoblot and enzyme linked immunosorbent assay (ELISA) against antigen preparations from two strains of S bovis and one strain of Enterococcus faecalis. RESULTS--No distinction between cancer patients and controls could be made using immunoblots. ELISA did show an increase in antibodies to S bovis, but there was a greater increase in antibodies to E faecalis. The increase in antibody titres was greatest with antigens which had been treated with periodate, and was therefore thought not to be caused by antibody to the shared group D carbohydrate antigen. CONCLUSION--It may be possible to construct a test for the detection of colonic cancer based on the detection of antibody to S bovis or E faecalis, though considerable further development of this concept is required.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beeching N. J., Christmas T. I., Ellis-Pegler R. B., Nicholson G. I. Streptococcus bovis bacteraemia requires rigorous exclusion of colonic neoplasia and endocarditis. Q J Med. 1985 Aug;56(220):439–450. [PubMed] [Google Scholar]
  2. Burnie J. P., Holland M., Matthews R. C., Lees W. Role of immunoblotting in the diagnosis of culture negative and enterococcal endocarditis. J Clin Pathol. 1987 Oct;40(10):1149–1158. doi: 10.1136/jcp.40.10.1149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Friedrich I. A., Wormser G. P., Gottfried E. B. The association of recent Streptococcus bovis bacteremia with colonic neoplasia. Mil Med. 1982 Jul;147(7):584–585. [PubMed] [Google Scholar]
  4. Kaplan M. H., Chmel H., Stephens A., Hsieh H. C., Tenenbaum M. J., Rothenberg I. R., Joachim G. R. Humoral reactions in human endocarditis due to Streptococcus bovis: evidence for a common S bovis antigen. J Infect Dis. 1983 Aug;148(2):266–274. doi: 10.1093/infdis/148.2.266. [DOI] [PubMed] [Google Scholar]
  5. Klein R. S., Catalano M. T., Edberg S. C., Casey J. I., Steigbigel N. H. Streptococcus bovis septicemia and carcinoma of the colon. Ann Intern Med. 1979 Oct;91(4):560–562. doi: 10.7326/0003-4819-91-4-560. [DOI] [PubMed] [Google Scholar]
  6. Klein R. S., Recco R. A., Catalano M. T., Edberg S. C., Casey J. I., Steigbigel N. H. Association of Streptococcus bovis with carcinoma of the colon. N Engl J Med. 1977 Oct 13;297(15):800–802. doi: 10.1056/NEJM197710132971503. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Morris E. J., Ganeshkumar N., McBride B. C. Cell surface components of Streptococcus sanguis: relationship to aggregation, adherence, and hydrophobicity. J Bacteriol. 1985 Oct;164(1):255–262. doi: 10.1128/jb.164.1.255-262.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Panwalker A. P. Unusual infections associated with colorectal cancer. Rev Infect Dis. 1988 Mar-Apr;10(2):347–364. doi: 10.1093/clinids/10.2.347. [DOI] [PubMed] [Google Scholar]
  11. Ruoff K. L., Miller S. I., Garner C. V., Ferraro M. J., Calderwood S. B. Bacteremia with Streptococcus bovis and Streptococcus salivarius: clinical correlates of more accurate identification of isolates. J Clin Microbiol. 1989 Feb;27(2):305–308. doi: 10.1128/jcm.27.2.305-308.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shanson D. C., Kirk N., Humphrey R. Clinical evaluation of a fluorescent antibody test for the serological diagnosis of streptococcal endocarditis. J Clin Pathol. 1985 Jan;38(1):92–98. doi: 10.1136/jcp.38.1.92. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. WICKEN A. J., BADDILEY J. Structure of intracellular teichoic acids from group D streptococci. Biochem J. 1963 Apr;87:54–62. doi: 10.1042/bj0870054. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Zarkin B. A., Lillemoe K. D., Cameron J. L., Effron P. N., Magnuson T. H., Pitt H. A. The triad of Streptococcus bovis bacteremia, colonic pathology, and liver disease. Ann Surg. 1990 Jun;211(6):786–792. doi: 10.1097/00000658-199006000-00019. [DOI] [PMC free article] [PubMed] [Google Scholar]

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