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. 1994 Feb;47(2):129–133. doi: 10.1136/jcp.47.2.129

Immunohistochemical study of vascular injury in acute multiple sclerosis.

A J Wakefield 1, L J More 1, J Difford 1, J E McLaughlin 1
PMCID: PMC501826  PMID: 8132826

Abstract

AIMS--To examine the vascular changes occurring in three archival cases of acute multiple sclerosis, and to provide immunohistochemical evidence of early endothelial cell activation and vascular occlusion in this condition. METHODS--Central nervous system tissues from three cases of acute active multiple sclerosis and six non-inflammatory controls were stained using the following methods: haematoxylin and eosin, Luxol fast blue, cresyl violet, Bielschowsky's silver, and reticulin. Tissues were also immunostained with specific antibodies against collagen type IV, factor XIIIa, class II antigens, glial fibrillary acidic protein, and fibrinogen. RESULTS--Early vascular endothelial cell activation which may progress to vasculitis and vascular occlusion including class II antigen expression and fibrin deposition were identified. The vascular changes were seen prior to cerebral parenchymal reaction and demyelination, and were not seen in control cerebral tissues. CONCLUSION--It is proposed that vascular endothelial cell activation may be an early and pivotal event in the evolution of multiple sclerosis, and that demyelination may have an ischaemic basis in this condition. The vascular endothelium may contain an early element in the evolution of multiple sclerosis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams C. W. Perivascular iron deposition and other vascular damage in multiple sclerosis. J Neurol Neurosurg Psychiatry. 1988 Feb;51(2):260–265. doi: 10.1136/jnnp.51.2.260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bevilacqua M. P., Pober J. S., Majeau G. R., Cotran R. S., Gimbrone M. A., Jr Interleukin 1 (IL-1) induces biosynthesis and cell surface expression of procoagulant activity in human vascular endothelial cells. J Exp Med. 1984 Aug 1;160(2):618–623. doi: 10.1084/jem.160.2.618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bevilacqua M. P., Schleef R. R., Gimbrone M. A., Jr, Loskutoff D. J. Regulation of the fibrinolytic system of cultured human vascular endothelium by interleukin 1. J Clin Invest. 1986 Aug;78(2):587–591. doi: 10.1172/JCI112613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Compston D. A. The dissemination of multiple sclerosis. The Langdon-Brown lecture 1989. J R Coll Physicians Lond. 1990 Jul;24(3):207–218. [PMC free article] [PubMed] [Google Scholar]
  5. Emeis J. J., Kooistra T. Interleukin 1 and lipopolysaccharide induce an inhibitor of tissue-type plasminogen activator in vivo and in cultured endothelial cells. J Exp Med. 1986 May 1;163(5):1260–1266. doi: 10.1084/jem.163.5.1260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gibson G. E., Shimada M., Blass J. P. Alterations in acetylcholine synthesis and cyclic nucleotides in mild cerebral hypoxia. J Neurochem. 1978 Oct;31(4):757–760. doi: 10.1111/j.1471-4159.1978.tb00107.x. [DOI] [PubMed] [Google Scholar]
  7. Graham E. M., Stanford M. R., Sanders M. D., Kasp E., Dumonde D. C. A point prevalence study of 150 patients with idiopathic retinal vasculitis: 1. Diagnostic value of ophthalmological features. Br J Ophthalmol. 1989 Sep;73(9):714–721. doi: 10.1136/bjo.73.9.714. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lightman S., McDonald W. I., Bird A. C., Francis D. A., Hoskins A., Batchelor J. R., Halliday A. M. Retinal venous sheathing in optic neuritis. Its significance for the pathogenesis of multiple sclerosis. Brain. 1987 Apr;110(Pt 2):405–414. doi: 10.1093/brain/110.2.405. [DOI] [PubMed] [Google Scholar]
  9. Mantovani A., Dejana E. Cytokines as communication signals between leukocytes and endothelial cells. Immunol Today. 1989 Nov;10(11):370–375. doi: 10.1016/0167-5699(89)90270-3. [DOI] [PubMed] [Google Scholar]
  10. Nachman R. L., Hajjar K. A., Silverstein R. L., Dinarello C. A. Interleukin 1 induces endothelial cell synthesis of plasminogen activator inhibitor. J Exp Med. 1986 Jun 1;163(6):1595–1600. doi: 10.1084/jem.163.6.1595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nawroth P. P., Handley D. A., Esmon C. T., Stern D. M. Interleukin 1 induces endothelial cell procoagulant while suppressing cell-surface anticoagulant activity. Proc Natl Acad Sci U S A. 1986 May;83(10):3460–3464. doi: 10.1073/pnas.83.10.3460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Paterson P. Y. Experimental allergic encephalomyelitis: role of fibrin deposition in immunopathogenesis of inflammation in rats. Fed Proc. 1976 Nov;35(13):2428–2434. [PubMed] [Google Scholar]
  13. Pober J. S. Warner-Lambert/Parke-Davis award lecture. Cytokine-mediated activation of vascular endothelium. Physiology and pathology. Am J Pathol. 1988 Dec;133(3):426–433. [PMC free article] [PubMed] [Google Scholar]
  14. Selmaj K., Nowak Z., Tchórzewski H. Multiple sclerosis: effect of myelin basic protein on interleukin 1, interleukin 2 production and interleukin 2 receptor expression in vitro. Clin Exp Immunol. 1988 Jun;72(3):428–433. [PMC free article] [PubMed] [Google Scholar]
  15. Sobel R. A., Blanchette B. W., Bhan A. K., Colvin R. B. The immunopathology of experimental allergic encephalomyelitis. II. Endothelial cell Ia increases prior to inflammatory cell infiltration. J Immunol. 1984 May;132(5):2402–2407. [PubMed] [Google Scholar]
  16. Wakefield A. J., Sankey E. A., Dhillon A. P., Sawyerr A. M., More L., Sim R., Pittilo R. M., Rowles P. M., Hudson M., Lewis A. A. Granulomatous vasculitis in Crohn's disease. Gastroenterology. 1991 May;100(5 Pt 1):1279–1287. [PubMed] [Google Scholar]
  17. Wakefield A. J., Sawyerr A. M., Dhillon A. P., Pittilo R. M., Rowles P. M., Lewis A. A., Pounder R. E. Pathogenesis of Crohn's disease: multifocal gastrointestinal infarction. Lancet. 1989 Nov 4;2(8671):1057–1062. doi: 10.1016/s0140-6736(89)91078-7. [DOI] [PubMed] [Google Scholar]

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