Abstract
A commercial latex slide test (Oxoid DR 620) for the identification of Escherichia coli O157 was compared with a standard tube agglutination method using E coli O157 antiserum (Difco). Thirty isolates previously confirmed as E coli O157 were positive by both methods: 30 isolates of E coli of serogroups other than O157, 30 isolates of non-sorbitol fermenting organisms from various genera, and one isolate of E hermanii were all negative by both methods. It is concluded that the latex slide test offers a rapid and economical alternative to tube agglutination for the identification of E coli O157.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chapman P. A., Mitchelmore D. L. A two-year survey of the incidence of heat-labile enterotoxin-producing Escherichia coli and other enteric pathogens in travellers returning to the Sheffield area. Epidemiol Infect. 1988 Oct;101(2):239–247. doi: 10.1017/s0950268800054157. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chapman P. A., Wright D. J., Norman P. Verotoxin-producing Escherichia coli infections in Sheffield: cattle as a possible source. Epidemiol Infect. 1989 Jun;102(3):439–445. doi: 10.1017/s0950268800030156. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karmali M. A., Petric M., Lim C., Fleming P. C., Arbus G. S., Lior H. The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli. J Infect Dis. 1985 May;151(5):775–782. doi: 10.1093/infdis/151.5.775. [DOI] [PubMed] [Google Scholar]
- Karmali M. A., Steele B. T., Petric M., Lim C. Sporadic cases of haemolytic-uraemic syndrome associated with faecal cytotoxin and cytotoxin-producing Escherichia coli in stools. Lancet. 1983 Mar 19;1(8325):619–620. doi: 10.1016/s0140-6736(83)91795-6. [DOI] [PubMed] [Google Scholar]
- Konowalchuk J., Dickie N., Stavric S., Speirs J. I. Properties of an Escherichia coli cytotoxin. Infect Immun. 1978 May;20(2):575–577. doi: 10.1128/iai.20.2.575-577.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lior H., Borczyk A. A. False positive identifications of Escherichia coli O157. Lancet. 1987 Feb 7;1(8528):333–333. doi: 10.1016/s0140-6736(87)92065-4. [DOI] [PubMed] [Google Scholar]
- March S. B., Ratnam S. Sorbitol-MacConkey medium for detection of Escherichia coli O157:H7 associated with hemorrhagic colitis. J Clin Microbiol. 1986 May;23(5):869–872. doi: 10.1128/jcm.23.5.869-872.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pai C. H., Gordon R., Sims H. V., Bryan L. E. Sporadic cases of hemorrhagic colitis associated with Escherichia coli O157:H7. Clinical, epidemiologic, and bacteriologic features. Ann Intern Med. 1984 Dec;101(6):738–742. doi: 10.7326/0003-4819-101-6-738. [DOI] [PubMed] [Google Scholar]
- Riley L. W., Remis R. S., Helgerson S. D., McGee H. B., Wells J. G., Davis B. R., Hebert R. J., Olcott E. S., Johnson L. M., Hargrett N. T. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N Engl J Med. 1983 Mar 24;308(12):681–685. doi: 10.1056/NEJM198303243081203. [DOI] [PubMed] [Google Scholar]
- Scotland S. M., Rowe B., Smith H. R., Willshaw G. A., Gross R. J. Vero cytotoxin-producing strains of Escherichia coli from children with haemolytic uraemic syndrome and their detection by specific DNA probes. J Med Microbiol. 1988 Apr;25(4):237–243. doi: 10.1099/00222615-25-4-237. [DOI] [PubMed] [Google Scholar]
- Smith H. R., Rowe B., Gross R. J., Fry N. K., Scotland S. M. Haemorrhagic colitis and Vero-cytotoxin-producing Escherichia coli in England and Wales. Lancet. 1987 May 9;1(8541):1062–1065. doi: 10.1016/s0140-6736(87)90485-5. [DOI] [PubMed] [Google Scholar]