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. 1989 Nov;42(11):1201–1205. doi: 10.1136/jcp.42.11.1201

Use of APAAP technique on paraffin wax embedded bone marrow trephines.

W N Erber 1, J McLachlan 1
PMCID: PMC501982  PMID: 2685054

Abstract

The immunoalkaline phosphatase (APAAP) technique was applied to the labelling of decalcified sections of formalin fixed, paraffin wax embedded bone marrow trephine biopsy specimens. A panel of monoclonal antibodies reactive with haemopoietic and epithelial antigens, which survive routine formalin fixation, was assessed on 72 cases of haematological malignancy (including acute and chronic leukaemias and lymphomas) showing bone marrow infiltration. The APAAP method showed clear distinct labelling of antigen positive cells without loss of antigens due to decalcification. Both normal or reactive single cells present in the sample and neoplastic cell populations could be identified morphologically and their antigenic phenotype and cellular origin, whether lymphoid or myeloid, established. The application of the APAAP method to routinely prepared paraffin wax embedded trephines has many advantages over the assessment of specially prepared cryostat sections of bone marrow.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Athanasou N. A., Quinn J., Heryet A., Woods C. G., McGee J. O. Effect of decalcification agents on immunoreactivity of cellular antigens. J Clin Pathol. 1987 Aug;40(8):874–878. doi: 10.1136/jcp.40.8.874. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cordell J. L., Falini B., Erber W. N., Ghosh A. K., Abdulaziz Z., MacDonald S., Pulford K. A., Stein H., Mason D. Y. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984 Feb;32(2):219–229. doi: 10.1177/32.2.6198355. [DOI] [PubMed] [Google Scholar]
  3. Davey F. R., Gatter K. C., Ralfkiaer E., Pulford K. A., Krissansen G. W., Mason D. Y. Immunophenotyping of non-Hodgkin's lymphomas using a panel of antibodies on paraffin-embedded tissues. Am J Pathol. 1987 Oct;129(1):54–63. [PMC free article] [PubMed] [Google Scholar]
  4. Erber W. N., Breton-Gorius J., Villeval J. L., Oscier D. G., Bai Y., Mason D. Y. Detection of cells of megakaryocyte lineage in haematological malignancies by immuno-alkaline phosphatase labelling cell smears with a panel of monoclonal antibodies. Br J Haematol. 1987 Jan;65(1):87–94. doi: 10.1111/j.1365-2141.1987.tb06140.x. [DOI] [PubMed] [Google Scholar]
  5. Erber W. N., Mynheer L. C., Mason D. Y. APAAP labelling of blood and bone-marrow samples for phenotyping leukaemia. Lancet. 1986 Apr 5;1(8484):761–765. doi: 10.1016/s0140-6736(86)91781-2. [DOI] [PubMed] [Google Scholar]
  6. Falini B., Martelli M. F., Tarallo F., Moir D. J., Cordell J. L., Gatter K. C., Loreti G., Stein H., Mason D. Y. Immunohistological analysis of human bone marrow trephine biopsies using monoclonal antibodies. Br J Haematol. 1984 Mar;56(3):365–386. doi: 10.1111/j.1365-2141.1984.tb03968.x. [DOI] [PubMed] [Google Scholar]
  7. Feller A. C., Wacker H. H., Moldenhauer G., Radzun H. J., Parwaresch M. R. Monoclonal antibody Ki-B3 detects a formalin resistant antigen on normal and neoplastic B cells. Blood. 1987 Sep;70(3):629–636. [PubMed] [Google Scholar]
  8. Gatter K. C., Cordell J. L., Turley H., Heryet A., Kieffer N., Anstee D. J., Mason D. Y. The immunohistological detection of platelets, megakaryocytes and thrombi in routinely processed specimens. Histopathology. 1988 Sep;13(3):257–267. doi: 10.1111/j.1365-2559.1988.tb02037.x. [DOI] [PubMed] [Google Scholar]
  9. Hall P. A., Lindeman R., Butler M. G., Amess J. A., D'Ardenne A. J. Demonstration of lymphoid antigens in decalcified bone marrow trephines. J Clin Pathol. 1987 Aug;40(8):870–873. doi: 10.1136/jcp.40.8.870. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mason D. Y., Gatter K. C. The role of immunocytochemistry in diagnostic pathology. J Clin Pathol. 1987 Sep;40(9):1042–1054. doi: 10.1136/jcp.40.9.1042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Norton A. J., Isaacson P. G. An immunocytochemical study of T-cell lymphomas using monoclonal and polyclonal antibodies effective in routinely fixed wax embedded tissues. Histopathology. 1986 Dec;10(12):1243–1260. doi: 10.1111/j.1365-2559.1986.tb02568.x. [DOI] [PubMed] [Google Scholar]
  12. Norton A. J., Isaacson P. G. Detailed phenotypic analysis of B-cell lymphoma using a panel of antibodies reactive in routinely fixed wax-embedded tissue. Am J Pathol. 1987 Aug;128(2):225–240. [PMC free article] [PubMed] [Google Scholar]
  13. Norton A. J., Ramsay A. D., Smith S. H., Beverley P. C., Isaacson P. G. Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues. J Clin Pathol. 1986 Apr;39(4):399–405. doi: 10.1136/jcp.39.4.399. [DOI] [PMC free article] [PubMed] [Google Scholar]

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