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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Virology. 2016 Jan 21;490:6–16. doi: 10.1016/j.virol.2015.10.013

Fig. 7.

Fig. 7

Modifications of Gag-iCre assay to study viral tropism and heterologous viral fusion glycoproteins. (A) Donor Jurkat cells were nucleofected with HIV Gag-iCre JRFL and co-cultured with the CCR5 expressing T cell Cre-responsive cell line, A3R5-RG for 48 h untreated or in presence of fusion antagonists, TAK779 or AMD3100. Entry efficiency was measured by flow cytometry. (B) Pseudotypted Gag-iCreΔEnv was produced by co transfection of HIV Gag-iCre with Ebola GP, Ebola mutant GP or VSV envelope. Cell free virus co-cultured with Hela RG target cell line for 48 hours. Entry efficiency was measured by flow cytometry.