Video 3. Disassembly of an in vitro generated ER network by cytATL.
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DOI: 10.7554/eLife.18605.010
An ER network was formed in a microfluidics chamber from Xenopus crude extract containing DiIC18. Xenopus egg cytosol containing 5 µM cytATL-GFP was slowly perfused into the chamber using a computer-controlled pneumatic pump (blue arrow indicates perfusion port) while images were acquired with a spinning disk confocal microscope at 10-sec intervals for 9 min. The video is shown at 3 frames per sec. Yellow arrowheads indicate ER disassembly events. Still images of this video were used for Figure 3. Scale bar = 5 µm.