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. 1990 Jan;43(1):43–45. doi: 10.1136/jcp.43.1.43

Changes in alveolar macrophage, monocyte, and neutrophil cell profiles after smoke inhalation injury.

B M Riyami 1, R Tree 1, J Kinsella 1, C J Clark 1, W H Reid 1, D Campbell 1, C G Gemmell 1
PMCID: PMC502222  PMID: 2312750

Abstract

Thirty two fire victims with smoke inhalation, with or without burns, and 26 control subjects had bronchoalveolar lavage performed. Cell yields and differential cell counts were assessed. All patients and controls were cigarette smokers. Patients with smoke inhalation (SI) injury generally showed higher total bronchoalveolar lavage (BAL) cell yields, and this was significant on repeat lavage from 12 patients. The increase was almost entirely due to an increase in the proportion of neutrophils in patients with smoke inhalation alone (S) and those with cutaneous burns as well as smoke inhalation (S + B). On sequential lavage of 12 patients with smoke inhalation (SI) the proportion of neutrophils had increased; this was significantly higher than on initial lavage. Using various macrophage markers, the proportions of macrophage subgroups were determined. There was an increase in UCHM1 and RFD9 positive cells in each subgroup: the increase in UCHM1 positive cells was significant in patients with burns as well as smoke inhalation, and the increase in RFD9 positive cells was significant in patients with smoke inhalation alone. Assessment of the role of such cells in the development of acute lung injury (such as adult respiratory distress syndrome) may be important in our understanding of the mechanisms entailed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Campbell D. A., Poulter L. W., Du Bois R. M. Phenotypic analysis of alveolar macrophages in normal subjects and in patients with interstitial lung disease. Thorax. 1986 Jun;41(6):429–434. doi: 10.1136/thx.41.6.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clark C. J. Measurement of toxic combustion products in fire survivors. J R Soc Med. 1982;75(Suppl 1):40–44. [PMC free article] [PubMed] [Google Scholar]
  3. Clark C. J., Pollock A. J., Reid W. H., Campbell D., Gemmell C. Role of pulmonary alveolar macrophage activation in acute lung injury after burns and smoke inhalation. Lancet. 1988 Oct 15;2(8616):872–874. doi: 10.1016/s0140-6736(88)92471-3. [DOI] [PubMed] [Google Scholar]
  4. Clark C. J., Reid W. H., Gilmour W. H., Campbell D. Mortality probability in victims of fire trauma: revised equation to include inhalation injury. Br Med J (Clin Res Ed) 1986 May 17;292(6531):1303–1305. doi: 10.1136/bmj.292.6531.1303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clark C. J., Reid W. H., Telfer A. B., Campbell D. Respiratory injury in the burned patient. The role of flexible bronchoscopy. Anaesthesia. 1983 Jan;38(1):35–39. doi: 10.1111/j.1365-2044.1983.tb10370.x. [DOI] [PubMed] [Google Scholar]
  6. Davis C. F., Moore F. D., Jr, Rodrick M. L., Fearon D. T., Mannick J. A. Neutrophil activation after burn injury: contributions of the classic complement pathway and of endotoxin. Surgery. 1987 Sep;102(3):477–484. [PubMed] [Google Scholar]
  7. Demarest G. B., Hudson L. D., Altman L. C. Impaired alveolar macrophage chemotaxis in patients with acute smoke inhalation. Am Rev Respir Dis. 1979 Feb;119(2):279–286. doi: 10.1164/arrd.1979.119.2.279. [DOI] [PubMed] [Google Scholar]
  8. Heideman M. The effect of thermal injury on hemodynamic, respiratory, and hematologic variables in relation to complement activation. J Trauma. 1979 Apr;19(4):239–247. doi: 10.1097/00005373-197904000-00004. [DOI] [PubMed] [Google Scholar]
  9. Hunninghake G. W., Gadek J. E., Fales H. M., Crystal R. G. Human alveolar macrophage-derived chemotactic factor for neutrophils. Stimuli and partial characterization. J Clin Invest. 1980 Sep;66(3):473–483. doi: 10.1172/JCI109878. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hunninghake G. W., Gadek J. E., Kawanami O., Ferrans V. J., Crystal R. G. Inflammatory and immune processes in the human lung in health and disease: evaluation by bronchoalveolar lavage. Am J Pathol. 1979 Oct;97(1):149–206. [PMC free article] [PubMed] [Google Scholar]
  11. Mason D. Y., Sammons R. Alkaline phosphatase and peroxidase for double immunoenzymatic labelling of cellular constituents. J Clin Pathol. 1978 May;31(5):454–460. doi: 10.1136/jcp.31.5.454. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. PHILLIPS A. W., COPE O. Burn therapy. II. The revelation of respiratory tract damage as a principal killer of the burned patient. Ann Surg. 1962 Jan;155:1–19. doi: 10.1097/00000658-196201000-00001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Reynolds H. Y. Bronchoalveolar lavage. Am Rev Respir Dis. 1987 Jan;135(1):250–263. doi: 10.1164/arrd.1987.135.1.250. [DOI] [PubMed] [Google Scholar]
  14. Rocker G. M., Wiseman M. S., Pearson D., Shale D. J. Diagnostic criteria for adult respiratory distress syndrome: time for reappraisal. Lancet. 1989 Jan 21;1(8630):120–123. doi: 10.1016/s0140-6736(89)91142-2. [DOI] [PubMed] [Google Scholar]
  15. Zawacki B. E., Jung R. C., Joyce J., Rincon E. Smoke, burns, and the natural history of inhalation injury in fire victims: a correlation of experimental and clinical data. Ann Surg. 1977 Jan;185(1):100–110. doi: 10.1097/00000658-197701000-00017. [DOI] [PMC free article] [PubMed] [Google Scholar]

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