. 2015 Oct 21;3(4):707–724. doi: 10.3390/microorganisms3040707

Table 1.

Polymerase chain reaction (PCR) primers used in this study.

Primer	Sequence 5ʹ→3ʹ	Target Gene	Amplicon Size	References
16s_27fw	agagtttgatcctggctcag	16S rDNA	~1.5 kb	Lane et al. 1991 [46]
16s_1492rv	gcctaccttgttacgactt	Bacteria	~1.5 kb	Lane et al. 1991 [46]
Arch25F	cyggttgatcctgccrg	18S rDNA	~1.5 kb	Achenbach and Woese 1995 [47]
Arch1492R	tacggytaccttgttacgactt	Archaea	~1.5 kb	Achenbach and Woese 1995 [47]
sorC1-F	Gtiggiccnaargtntgy *	Sor	~230 bp	Chen et al. 2007 [41]
sorH1-R	rtgcatntcytcrtgrtc	Sor	~230 bp	Chen et al. 2007 [41]
bsor_1F	gtccttcgagaccatgatgmargtnggncc	bacterialsor (CODEHOP)	~800 bp	This study
bsor_2R	ccgccactgggcctsytccatcatng	bacterialsor (CODEHOP)	~800 bp	This study
PCJ2_for	caggcctcccagcaggtnggnccnaa	sor(CODEHOP)	840 bp	This study
PCJ3_rev	ctcccgccatgaggtgtcctccatnayngg	sor(CODEHOP)	840 bp	This study
SULFO170F	caatcccgcatacgttcc	16S rDNA	436 bp	De Wulf-Durand et al. 1997 [45]
SULFO606R	aaaccgctacgtatcgcac	Sulfobacillus spp.	436 bp	De Wulf-Durand et al. 1997 [45]
CALD460F	atccgaatacggtctgcta	16S rDNA	~1 kb	De Wulf-Durand et al. 1997 [45]
CALD1475R	tataccgtggtcgtcgcc	At. caldus	~1 kb	De Wulf-Durand et al. 1997 [45]
THIO458F	gggtgctaatawcgcctgctg	16S rDNA	~1 kb	De Wulf-Durand et al. 1997 [45]
THIO1473R	taccgtggtcatcgccct	At. thiooxidans	~1 kb	De Wulf-Durand et al. 1997 [45]
LEPTO176F	cgaatagtatccggttccg	16S rDNA	503 bp	De Wulf-Durand et al. 1997 [45]
LEPTO679R	aaattccgcttccctctcc	Leptospirillumspp.	503 bp	De Wulf-Durand et al. 1997 [45]
FERRO458F	gggttctaatacaatctgct	16S rDNA	~1 kb	De Wulf-Durand et al. 1997 [45]
FERRO1473	taccgtggtaaccgccct	At. ferrooxidans	~1 kb	De Wulf-Durand et al. 1997 [45]
T7	taatacgactcactataggg	Promoterregions	158 bp	Promega® pGEM-T vector manual
SP6	atttaggtgacactatagaa	in pGEM®-T vector	158 bp	Promega® pGEM-T vector manual

* i, inosine.