Table 3.

Overview of the most recent advances for the recovery and identification of Alicyclobacillus spp. (2014 and 2015).

Method	Description	Reference
Lipase and esterase fingerprints	Juice incubation at 45 °C for 24 h, cell harvesting and chromatography	[60]
Aptamer-based enrichment 16S rDNA	The method requires a preliminary enrichment step, so it can take up to 1 week. After a mechanical treatment, DNA was quantified through a RT-PCR based approach	[61]
Immunomagnetic separation RT-PCR	Immunomagnetic separation was combined with RT-PCR, by using two probes. The method is highly selective for A. acidoterrestris	[38]
FIR	Fourier transformed intra-red spectroscopy (1350–1700/cm), combined with multivariate statistical analysis (Principal Component Analysis and Class Analogy), allows the discrimination between Bacillus and Alicyclobacillus spp.	[62]
G-quadruplex colorimetric method	A. acidoterrestris was grown at 45 °C in presence of vanillic acid; this compound is easily converted to guaiacol and finally to tetraguaiacol (amber-coloured). The reaction is catalysed by G-quadruplex DNA-zyme	[63]
DAS-ELISA	DAS-ELISA (double antibodies sandwich ELISA) assay is based on the two kinds of polyclonal antibodies from Japanese White rabbit. The method shows high sensitivity and excellent agreement with isolation by K medium	[64]