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. 1990 Apr;43(4):325–328. doi: 10.1136/jcp.43.4.325

Proliferation in non-Hodgkin's lymphoma: a comparison of Ki-67 staining on fine needle aspiration and cryostat sections.

D C Brown 1, K C Gatter 1, D Y Mason 1
PMCID: PMC502371  PMID: 2341568

Abstract

Assessment of the growth fraction of non-Hodgkin's lymphomas may provide useful additional prognostic information to that obtained with conventional histological criteria. The monoclonal antibody Ki-67 has been reported to provide such information immunocytochemically in tissue biopsy specimens from lymphoma as well as other tumours. This study was undertaken to assess whether this approach could be extended to fine needle aspiration (FNA) biopsy specimens which are becoming increasingly important in the diagnosis of lymphoma. In 21 cases of non-Hodgkin's lymphoma the rate of tumour proliferation estimated by Ki-67 immunostaining of FNA material, obtained from surgically removed specimens, was compared with that obtained on tissue biopsy. The correlation between both preparations was excellent, indicating that FNA biopsy material is suitable for the immunocytochemical assessment of the growth fraction of non-Hodgkin's lymphoma.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnard N. J., Hall P. A., Lemoine N. R., Kadar N. Proliferative index in breast carcinoma determined in situ by Ki67 immunostaining and its relationship to clinical and pathological variables. J Pathol. 1987 Aug;152(4):287–295. doi: 10.1002/path.1711520407. [DOI] [PubMed] [Google Scholar]
  2. Betsill W. L., Jr, Hajdu S. I. Percutaneous aspiration biopsy of lymph nodes. Am J Clin Pathol. 1980 Apr;73(4):471–479. doi: 10.1093/ajcp/73.4.471. [DOI] [PubMed] [Google Scholar]
  3. Braylan R. C., Diamond L. W., Powell M. L., Harty-Golder B. Percentage of cells in the S phase of the cell cycle in human lymphoma determined by flow cytometry. Cytometry. 1980 Nov;1(3):171–174. doi: 10.1002/cyto.990010302. [DOI] [PubMed] [Google Scholar]
  4. Brown D. C., Cole D., Gatter K. C., Mason D. Y. Carcinoma of the cervix uteri: an assessment of tumour proliferation using the monoclonal antibody Ki67. Br J Cancer. 1988 Feb;57(2):178–181. doi: 10.1038/bjc.1988.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brown D. C., Gatter K. C., Dunnill M. S., Mason D. Y. Immunocytochemical analysis of cytocentrifuged fine needle aspirates. A study based on lung tumors aspirated in vitro. Anal Quant Cytol Histol. 1989 Apr;11(2):140–145. [PubMed] [Google Scholar]
  6. Cordell J. L., Falini B., Erber W. N., Ghosh A. K., Abdulaziz Z., MacDonald S., Pulford K. A., Stein H., Mason D. Y. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984 Feb;32(2):219–229. doi: 10.1177/32.2.6198355. [DOI] [PubMed] [Google Scholar]
  7. Gatter K. C., Dunnill M. S., Gerdes J., Stein H., Mason D. Y. New approach to assessing lung tumours in man. J Clin Pathol. 1986 Jun;39(6):590–593. doi: 10.1136/jcp.39.6.590. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gerdes J., Dallenbach F., Lennert K., Lemke H., Stein H. Growth fractions in malignant non-Hodgkin's lymphomas (NHL) as determined in situ with the monoclonal antibody Ki-67. Hematol Oncol. 1984 Oct-Dec;2(4):365–371. doi: 10.1002/hon.2900020406. [DOI] [PubMed] [Google Scholar]
  9. Gerdes J., Dallenbach F., Lennert K., Lemke H., Stein H. Growth fractions in malignant non-Hodgkin's lymphomas (NHL) as determined in situ with the monoclonal antibody Ki-67. Hematol Oncol. 1984 Oct-Dec;2(4):365–371. doi: 10.1002/hon.2900020406. [DOI] [PubMed] [Google Scholar]
  10. Gerdes J., Lemke H., Baisch H., Wacker H. H., Schwab U., Stein H. Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 1984 Oct;133(4):1710–1715. [PubMed] [Google Scholar]
  11. Gerdes J., Schwab U., Lemke H., Stein H. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 1983 Jan 15;31(1):13–20. doi: 10.1002/ijc.2910310104. [DOI] [PubMed] [Google Scholar]
  12. Habeshaw J. A., Lister T. A., Stansfeld A. G., Greaves M. F. Correlation of transferrin receptor expression with histological class and outcome in non-Hodgkin lymphoma. Lancet. 1983 Mar 5;1(8323):498–501. doi: 10.1016/s0140-6736(83)92191-8. [DOI] [PubMed] [Google Scholar]
  13. Hall P. A., Richards M. A., Gregory W. M., d'Ardenne A. J., Lister T. A., Stansfeld A. G. The prognostic value of Ki67 immunostaining in non-Hodgkin's lymphoma. J Pathol. 1988 Mar;154(3):223–235. doi: 10.1002/path.1711540305. [DOI] [PubMed] [Google Scholar]
  14. Kvaløy S., Godal T., Marton P. F., Steen H., Brenhovd I. O., Abrahamsen A. F. Spontaneous (H)-thymidine uptake in histological subgroups of human B-cell lymphomas. Scand J Haematol. 1981 Mar;26(3):221–234. doi: 10.1111/j.1600-0609.1981.tb01650.x. [DOI] [PubMed] [Google Scholar]
  15. Levitt S., Cheng L., DuPuis M. H., Layfield L. J. Fine needle aspiration diagnosis of malignant lymphoma with confirmation by immunoperoxidase staining. Acta Cytol. 1985 Sep-Oct;29(5):895–902. [PubMed] [Google Scholar]
  16. Martin S. E., Zhang H. Z., Magyarosy E., Jaffe E. S., Hsu S. M., Chu E. W. Immunologic methods in cytology: definitive diagnosis of non-Hodgkin's lymphomas using immunologic markers for T- and B-cells. Am J Clin Pathol. 1984 Dec;82(6):666–673. doi: 10.1093/ajcp/82.6.666. [DOI] [PubMed] [Google Scholar]
  17. Meyer J. S. Cell kinetic measurements of human tumors. Hum Pathol. 1982 Oct;13(10):874–877. doi: 10.1016/s0046-8177(82)80045-2. [DOI] [PubMed] [Google Scholar]
  18. National Cancer Institute sponsored study of classifications of non-Hodgkin's lymphomas: summary and description of a working formulation for clinical usage. The Non-Hodgkin's Lymphoma Pathologic Classification Project. Cancer. 1982 May 15;49(10):2112–2135. doi: 10.1002/1097-0142(19820515)49:10<2112::aid-cncr2820491024>3.0.co;2-2. [DOI] [PubMed] [Google Scholar]
  19. Oertel J., Oertel B., Kastner M., Lobeck H., Huhn D. The value of immunocytochemical staining of lymph node aspirates in diagnostic cytology. Br J Haematol. 1988 Nov;70(3):307–316. doi: 10.1111/j.1365-2141.1988.tb02487.x. [DOI] [PubMed] [Google Scholar]
  20. Pileri S., Gerdes J., Rivano M., Tazzari P. L., Magnani M., Gobbi M., Stein H. Immunohistochemical determination of growth fractions in human permanent cell lines and lymphoid tumours: a critical comparison of the monoclonal antibodies OKT9 and Ki-67. Br J Haematol. 1987 Mar;65(3):271–276. doi: 10.1111/j.1365-2141.1987.tb06853.x. [DOI] [PubMed] [Google Scholar]
  21. Schrape S., Jones D. B., Wright D. H. A comparison of three methods for the determination of the growth fraction in non-Hodgkin's lymphoma. Br J Cancer. 1987 Mar;55(3):283–286. doi: 10.1038/bjc.1987.54. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schwartz B. R., Pinkus G., Bacus S., Toder M., Weinberg D. S. Cell proliferation in non-Hodgkin's lymphomas. Digital image analysis of Ki-67 antibody staining. Am J Pathol. 1989 Feb;134(2):327–336. [PMC free article] [PubMed] [Google Scholar]
  23. Silvestrini R., Piazza R., Riccardi A., Rilke F. Correlation of cell kinetic findings with morphology of non-Hodgkin's malignant lymphomas. J Natl Cancer Inst. 1977 Mar;58(3):499–504. doi: 10.1093/jnci/58.3.499. [DOI] [PubMed] [Google Scholar]
  24. Stansfeld A. G., Diebold J., Noel H., Kapanci Y., Rilke F., Kelényi G., Sundstrom C., Lennert K., van Unnik J. A., Mioduszewska O. Updated Kiel classification for lymphomas. Lancet. 1988 Feb 6;1(8580):292–293. doi: 10.1016/s0140-6736(88)90367-4. [DOI] [PubMed] [Google Scholar]

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