Abstract
This short report serves as a warning to the unwary of possible "pseudoclusters" of infection with Aspergillus fumigatus as shown by the typing system, random amplification of polymorphic DNA (RAPD). This was demonstrated by typing 10 epidemiologically distinct isolates of A fumigatus using two different preparations of Taq DNA polymerase. One of the enzymes did not discriminate between the isolates, giving the false impression that a cluster of infection had occurred. Enzyme source is thus a key variable when using RAPD to distinguish between isolates of A fumigatus.
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- Aufauvre-Brown A., Cohen J., Holden D. W. Use of randomly amplified polymorphic DNA markers to distinguish isolates of Aspergillus fumigatus. J Clin Microbiol. 1992 Nov;30(11):2991–2993. doi: 10.1128/jcm.30.11.2991-2993.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Denning D. W., Stevens D. A. Antifungal and surgical treatment of invasive aspergillosis: review of 2,121 published cases. Rev Infect Dis. 1990 Nov-Dec;12(6):1147–1201. doi: 10.1093/clinids/12.6.1147. [DOI] [PubMed] [Google Scholar]
- Gerson S. L., Talbot G. H., Hurwitz S., Strom B. L., Lusk E. J., Cassileth P. A. Prolonged granulocytopenia: the major risk factor for invasive pulmonary aspergillosis in patients with acute leukemia. Ann Intern Med. 1984 Mar;100(3):345–351. doi: 10.7326/0003-4819-100-3-345. [DOI] [PubMed] [Google Scholar]
- Girardin H., Latgé J. P., Srikantha T., Morrow B., Soll D. R. Development of DNA probes for fingerprinting Aspergillus fumigatus. J Clin Microbiol. 1993 Jun;31(6):1547–1554. doi: 10.1128/jcm.31.6.1547-1554.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Girardin H., Sarfati J., Traoré F., Dupouy Camet J., Derouin F., Latgé J. P. Molecular epidemiology of nosocomial invasive aspergillosis. J Clin Microbiol. 1994 Mar;32(3):684–690. doi: 10.1128/jcm.32.3.684-690.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loudon K. W., Burnie J. P., Coke A. P., Matthews R. C. Application of polymerase chain reaction to fingerprinting Aspergillus fumigatus by random amplification of polymorphic DNA. J Clin Microbiol. 1993 May;31(5):1117–1121. doi: 10.1128/jcm.31.5.1117-1121.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loudon K. W., Coke A. P., Burnie J. P., Lucas G. S., Liu Yin J. A. Invasive aspergillosis: clusters and sources? J Med Vet Mycol. 1994;32(3):217–224. doi: 10.1080/02681219480000281. [DOI] [PubMed] [Google Scholar]
- Schierwater B., Ender A. Different thermostable DNA polymerases may amplify different RAPD products. Nucleic Acids Res. 1993 Sep 25;21(19):4647–4648. doi: 10.1093/nar/21.19.4647. [DOI] [PMC free article] [PubMed] [Google Scholar]