Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1990 May;43(5):379–384. doi: 10.1136/jcp.43.5.379

Detection of a mucin marker for the adenoma-carcinoma sequence inhuman colonic mucosa by monoclonal antibody AM-3.

C Hanski 1, G Bornhoeft 1, N Topf 1, U Hermann 1, H Stein 1, E O Riecken 1
PMCID: PMC502434  PMID: 1695227

Abstract

The monoclonal antibody AM-3 was raised against mucins extracted from human colorectal carcinomas. It reacted strongly with sections of paraffin wax embedded colorectal carcinoma. In colonic adenoma tissue the percentage of cells expressing the epitope detected by AM-3 correlated with the degree of dysplasia. In contrast to immunohistochemical staining, which did not show the presence of the antigen in histologically normal mucosa, the more sensitive enzyme linked immunosorbent assay (ELISA) and immunoblot assays showed that it was weakly expressed in this tissue. AM-3 reacted with variable frequency with several normal and malignant human tissues, indicating that the detected epitope is not restricted to colonic tissue. In colonic carcinomas it is present on a sialomucin of apparent relative molecular mass of more than 440,000. These data suggest that the antigen detectable with AM-3 may be useful in the assessment of premalignant changes in colonic adenomas.

Full text

PDF
379

Images in this article

380Image
on p.380
381Image
on p.381
381Image
on p.381
382Image
on p.382

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe M., Kufe D. W. Identification of a family of high molecular weight tumor-associated glycoproteins. J Immunol. 1987 Jul 1;139(1):257–261. [PubMed] [Google Scholar]
  2. Atkinson B. F., Ernst C. S., Herlyn M., Steplewski Z., Sears H. F., Koprowski H. Gastrointestinal cancer-associated antigen in immunoperoxidase assay. Cancer Res. 1982 Nov;42(11):4820–4823. [PubMed] [Google Scholar]
  3. Boland C. R., Montgomery C. K., Kim Y. S. Alterations in human colonic mucin occurring with cellular differentiation and malignant transformation. Proc Natl Acad Sci U S A. 1982 Mar;79(6):2051–2055. doi: 10.1073/pnas.79.6.2051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boland C. R. Mucin histochemistry in colonic polyps and cancer. Semin Surg Oncol. 1987;3(3):183–189. doi: 10.1002/ssu.2980030311. [DOI] [PubMed] [Google Scholar]
  5. Filipe M. I., Barbatis C., Sandey A., Ma J. Expression of intestinal mucin antigens in the gastric epithelium and its relationship with malignancy. Hum Pathol. 1988 Jan;19(1):19–26. doi: 10.1016/s0046-8177(88)80311-3. [DOI] [PubMed] [Google Scholar]
  6. Friedman E., Thor A., Hand P. H., Schlom J. Surface expression of tumor-associated antigens in primary cultured human colonic epithelial cells from carcinomas, benign tumors, and normal tissues. Cancer Res. 1985 Nov;45(11 Pt 2):5648–5655. [PubMed] [Google Scholar]
  7. Gangopadhyay A., Bhattacharya M., Chatterjee S. K., Barlow J. J., Tsukada Y. Immunoperoxidase localization of a high-molecular-weight mucin recognized by monoclonal antibody 1D3. Cancer Res. 1985 Apr;45(4):1744–1752. [PubMed] [Google Scholar]
  8. Griffioen G., Bosman F. T., Verspaget H. W., de Bruin P. A., Biemond I., Lamers C. B. Mucin profiles and potential for malignancy of human colorectal adenomatous polyps. Cancer. 1989 Apr 15;63(8):1587–1591. doi: 10.1002/1097-0142(19890415)63:8<1587::aid-cncr2820630824>3.0.co;2-5. [DOI] [PubMed] [Google Scholar]
  9. Habib N. A., Dawson P. M., Bradfield J. W., Williamson R. C., Wood C. B. Sialomucins at resection margin and likelihood of recurrence in colorectal carcinoma. Br Med J (Clin Res Ed) 1986 Aug 30;293(6546):521–523. doi: 10.1136/bmj.293.6546.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hilkens J., Buijs F., Hilgers J., Hageman P., Calafat J., Sonnenberg A., van der Valk M. Monoclonal antibodies against human milk-fat globule membranes detecting differentiation antigens of the mammary gland and its tumors. Int J Cancer. 1984 Aug 15;34(2):197–206. doi: 10.1002/ijc.2910340210. [DOI] [PubMed] [Google Scholar]
  11. Konishi F., Morson B. C. Pathology of colorectal adenomas: a colonoscopic survey. J Clin Pathol. 1982 Aug;35(8):830–841. doi: 10.1136/jcp.35.8.830. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kufe D., Inghirami G., Abe M., Hayes D., Justi-Wheeler H., Schlom J. Differential reactivity of a novel monoclonal antibody (DF3) with human malignant versus benign breast tumors. Hybridoma. 1984 Fall;3(3):223–232. doi: 10.1089/hyb.1984.3.223. [DOI] [PubMed] [Google Scholar]
  13. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Maeda T., Balakrishnan K., Mehdi S. Q. A simple and rapid method for the preparation of plasma membranes. Biochim Biophys Acta. 1983 May 26;731(1):115–120. doi: 10.1016/0005-2736(83)90404-2. [DOI] [PubMed] [Google Scholar]
  16. Magnani J. L., Steplewski Z., Koprowski H., Ginsburg V. Identification of the gastrointestinal and pancreatic cancer-associated antigen detected by monoclonal antibody 19-9 in the sera of patients as a mucin. Cancer Res. 1983 Nov;43(11):5489–5492. [PubMed] [Google Scholar]
  17. McGee J. O., Woods J. C., Ashall F., Bramwell M. E., Harris H. A new marker for human cancer cells, 2 immunohistochemical detection of the Ca antigen in human tissues with the Ca1 antibody. Lancet. 1982 Jul 3;2(8288):7–10. doi: 10.1016/s0140-6736(82)91151-5. [DOI] [PubMed] [Google Scholar]
  18. Muto T., Bussey H. J., Morson B. C. The evolution of cancer of the colon and rectum. Cancer. 1975 Dec;36(6):2251–2270. doi: 10.1002/cncr.2820360944. [DOI] [PubMed] [Google Scholar]
  19. Schlom J., Colcher D., Hand P. H., Greiner J., Wunderlich D., Weeks M., Fisher P. B., Noguchi P., Pestka S., Kufe D. Monoclonal antibodies reactive with breast tumor-associated antigens. Adv Cancer Res. 1985;43:143–173. doi: 10.1016/s0065-230x(08)60945-8. [DOI] [PubMed] [Google Scholar]
  20. Stramignoni D., Bowen R., Atkinson B. F., Schlom J. Differential reactivity of monoclonal antibodies with human colon adenocarcinomas and adenomas. Int J Cancer. 1983 May 15;31(5):543–552. doi: 10.1002/ijc.2910310504. [DOI] [PubMed] [Google Scholar]
  21. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yonezawa S., Nakamura T., Tanaka S., Sato E. Glycoconjugate with Ulex europaeus agglutinin-I-binding sites in normal mucosa, adenoma, and carcinoma of the human large bowel. J Natl Cancer Inst. 1982 Oct;69(4):777–785. [PubMed] [Google Scholar]
  23. Yuan M., Itzkowitz S. H., Boland C. R., Kim Y. D., Tomita J. T., Palekar A., Bennington J. L., Trump B. F., Kim Y. S. Comparison of T-antigen expression in normal, premalignant, and malignant human colonic tissue using lectin and antibody immunohistochemistry. Cancer Res. 1986 Sep;46(9):4841–4847. [PubMed] [Google Scholar]
  24. Zotter S., Lossnitzer A., Hageman P. C., Delemarre J. F., Hilkens J., Hilgers J. Immunohistochemical localization of the epithelial marker MAM-6 in invasive malignancies and highly dysplastic adenomas of the large intestine. Lab Invest. 1987 Aug;57(2):193–199. [PubMed] [Google Scholar]
  25. Zotter S., Lossnitzer A., Kunze K. D., Müller M., Hilkens J., Hilgers J., Hageman P. Epithelial markers for paraffin-embedded human tissues. Immunohistochemistry with monoclonal antibodies against milk fat globule antigens. Virchows Arch A Pathol Anat Histopathol. 1985;406(2):237–251. doi: 10.1007/BF00737089. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES