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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Biomaterials. 2016 Aug 17;106:167–179. doi: 10.1016/j.biomaterials.2016.08.024

Figure 4. Dental CSs interaction in in vitro cultured GelMA constructs.

Figure 4

High magnification H&E images and IHC analyses of multilayered DE DM CSs GelMA constructs cultured in osteogenic media for 24 h and 4 days, stained with FAK, TEN and SYN4. Arrows indicate expression. Cell sheets are identified as epithelial (DE) and mesenchymal (DM). A. H&E stained DE DM CSs GelMA constructs cultured in osteogenic media for 24 h. FAK, TEN and SYN4 staining (B, C and D) were detected in the DM CSs cultured in osteogenic media for 24 h. E. H&E image of DE DM CSs GelMA constructs cultured in osteogenic media for 4 days. F. FAK staining was detected in DE and DM CSs cultured in osteogenic media for 4 days. G. TEN was detected in the DM CSs cultured in osteogenic media for 4 days. H. Faint SYN4 staining was detected in DM CSs cultured in osteogenic media for 4 days. I. No staining was detected in the negative controls. Specific staining was detected on the natural tooth bud (J. FAK, K. TEN and L. SYN4).