Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1990 Jul;43(7):587–590. doi: 10.1136/jcp.43.7.587

Evaluation of Cathra system for identifying gram negative aerobic bacteria.

J M Ling 1, L C Zhang 1, Y W Hui 1, G L French 1
PMCID: PMC502587  PMID: 2199538

Abstract

The Cathra system is a commercial multipoint inoculation method for the identification of aerobic Gram negative bacteria. The system uses a replicator technique in which 21 different agar media can be inoculated simultaneously with 36 organisms. Identifications are made by use of a special computer database. The performance of this system was compared with that of the API 20E for the identification of 372 clinical isolates of Enterobacteriaceae and 133 miscellaneous Gram negative bacteria. For enterobacteria, the Cathra system was in 97% agreement with API 20E at species level and 98% at genus level. For miscellaneous Gram negative strains the two systems were in 59% agreement at species level and 77% at genus level. The Cathra system is suitable for use in diagnostic laboratories, especially those with a heavy workload and a wish to use break-point sensitivity testing. The identification database for miscellaneous Gram negative organisms, however, needs to be expanded.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appelbaum P. C., Arthur R. R., Parker M. E., Shugar G. L., von Kuster L. C., Charache P. Comparison of three methods for identification of Enterobacteriaceae. Eur J Clin Microbiol. 1982 Apr;1(2):76–81. doi: 10.1007/BF02014195. [DOI] [PubMed] [Google Scholar]
  2. Barr J. G., Hogg G. M., Smyth E. T., Emmerson A. M. Comparison of identification of Enterobacteriaceae by API 20E and Sensititre Autoidentification System. J Clin Pathol. 1989 Jun;42(6):649–652. doi: 10.1136/jcp.42.6.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bennett C. H., Joynson D. H. Kit systems for identifying gram negative aerobic bacilli: report of the Welsh Standing Specialist Advisory Working Group in Microbiology. J Clin Pathol. 1986 Jun;39(6):666–671. doi: 10.1136/jcp.39.6.666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown S. D., Washington J. A., 2nd Evaluation of the Repliscan system for identification of Enterobacteriaceae. J Clin Microbiol. 1978 Dec;8(6):695–699. doi: 10.1128/jcm.8.6.695-699.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Castillo C. B., Bruckner D. A. Comparative evaluation of the Eiken and API 20E systems and conventional methods for identification of members of the family Enterobacteriaceae. J Clin Microbiol. 1984 Oct;20(4):754–757. doi: 10.1128/jcm.20.4.754-757.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Farmer J. J., 3rd, Davis B. R., Hickman-Brenner F. W., McWhorter A., Huntley-Carter G. P., Asbury M. A., Riddle C., Wathen-Grady H. G., Elias C., Fanning G. R. Biochemical identification of new species and biogroups of Enterobacteriaceae isolated from clinical specimens. J Clin Microbiol. 1985 Jan;21(1):46–76. doi: 10.1128/jcm.21.1.46-76.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fenn J. P., Matsen J. M. Packaged commercial bacterial identification systems. Clin Lab Med. 1985 Mar;5(1):19–58. [PubMed] [Google Scholar]
  8. Keville M. W., Doern G. V. Evaluation of the DMS Rapid E system for identification of clinical isolates of the family Enterobacteriaceae. J Clin Microbiol. 1984 Nov;20(5):1010–1011. doi: 10.1128/jcm.20.5.1010-1011.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kämpfer P., Dott W. Evaluation of the Titertek-NF system for identification of gram-negative nonfermentative and oxidase-positive fermentative bacteria. J Clin Microbiol. 1989 Jun;27(6):1201–1205. doi: 10.1128/jcm.27.6.1201-1205.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ling J. M., Hui Y. W., French G. L. Evaluation of the Microbact-24E bacterial identification system. J Clin Pathol. 1988 Aug;41(8):910–914. doi: 10.1136/jcp.41.8.910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Søgaard P., Gahrn-Hansen B., Zhou H. P., Frederiksen W. An investigation of three commercial methods for rapid identification of non-enteric gram-negative rods. Application on Pseudomonas paucimobilis and some other Pseudomonas species. Acta Pathol Microbiol Immunol Scand B. 1986 Oct;94(5):357–363. doi: 10.1111/j.1699-0463.1986.tb03067.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES