Figure 5.

Access of HIV-2 to Mature DC-Mediated Trans Infection Pathway is Diminished. Mature DCs (A) or activated CD4⁺ T cells (B) were pulsed with either HIV-1 (Lai-YU2) or HIV-2 (CDC77618) at an MOI=0.1. Virus-exposed mature DCs were washed to remove unbound virus and co-cultured with autologous CD4⁺ T cells. Mature DC-T cell co-culture supernatants or CD4⁺ T cell supernatants were harvested on day 6 post-infection and added to TZM-bl cells. The data shown is luciferase activity (RLU) in TZM-Bl cell lysates harvested 48 hr post initiation of infection and is the mean ±SEM from 4 experiments derived from cells from 4 independent donors. (C) Mature DCs were infected with GFP expressing single cycle replication competent viruses (pseudotyped with HIV-1/BaL Env or HIV-2 ST Env) at MOI=5. Virus-exposed mDCs were washed and cultured with autologous CD4⁺ T cells. Additionally, CD4⁺ T cells were infected directly (cell-free infection) in parallel. The percentage of GFP positive CD4⁺ T cells was quantified via flow cytometry. The data from 6 independent infections with cells from 6 donors is shown (mean ±SEM). *p=0.01