Abstract
AIMS--To develop a polymerase chain reaction (PCR) assay for the detection of cytomegalovirus (CMV) DNA in serum and leucocytes of renal transplant recipients and compare this assay with CMV culture and serodiagnosis. METHODS--Monthly specimens were obtained from 12 patients starting immediately before transplant. CMV infection was monitored by IgM enzyme linked immunosorbent assay, virus culture and PCR on serum and leucocytes. RESULTS--Two of four IgG positive patients had reactivation of CMV disease confirmed by culture, three of eight seronegative patients had a primary infection, one confirmed by serology and two by culture. PCR was positive earlier than conventional methods in three cases and concurrently in two. No positive PCR reactions occurred in the seven patients who remained negative by culture and serology. CONCLUSIONS--CMV DNA is detectable in serum; serum may be positive before virus is detectable by buffy coat culture; and PCR may be useful as an early indication of potential CMV disease in renal transplant recipients.
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Selected References
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- Brytting M., Xu W., Wahren B., Sundqvist V. A. Cytomegalovirus DNA detection in sera from patients with active cytomegalovirus infections. J Clin Microbiol. 1992 Aug;30(8):1937–1941. doi: 10.1128/jcm.30.8.1937-1941.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cinque P., Vago L., Brytting M., Castagna A., Accordini A., Sundqvist V. A., Zanchetta N., Monforte A. D., Wahren B., Lazzarin A. Cytomegalovirus infection of the central nervous system in patients with AIDS: diagnosis by DNA amplification from cerebrospinal fluid. J Infect Dis. 1992 Dec;166(6):1408–1411. doi: 10.1093/infdis/166.6.1408. [DOI] [PubMed] [Google Scholar]
- Kwok S., Higuchi R. Avoiding false positives with PCR. Nature. 1989 May 18;339(6221):237–238. doi: 10.1038/339237a0. [DOI] [PubMed] [Google Scholar]
- Prösch S., Kimel V., Dawydowa I., Krüger D. H. Monitoring of patients for cytomegalovirus after organ transplantation by centrifugation culture and PCR. J Med Virol. 1992 Dec;38(4):246–251. doi: 10.1002/jmv.1890380404. [DOI] [PubMed] [Google Scholar]
- Ratnamohan V. M., Mathÿs J. M., McKenzie A., Cunningham A. L. HCMV-DNA is detected more frequently than infectious virus in blood leucocytes of immunocompromised patients: a direct comparison of culture-immunofluorescence and PCR for detection of HCMV in clinical specimens. J Med Virol. 1992 Dec;38(4):252–259. doi: 10.1002/jmv.1890380405. [DOI] [PubMed] [Google Scholar]
- Rice P. S., Kudesia G., Price C., Smith G. H. Diagnosis of cytomegalovirus infection in heart transplant recipients. J Clin Pathol. 1992 Jul;45(7):636–637. doi: 10.1136/jcp.45.7.636. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stanier P., Taylor D. L., Kitchen A. D., Wales N., Tryhorn Y., Tyms A. S. Persistence of cytomegalovirus in mononuclear cells in peripheral blood from blood donors. BMJ. 1989 Oct 7;299(6704):897–898. doi: 10.1136/bmj.299.6704.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wakefield A. J., Fox J. D., Sawyerr A. M., Taylor J. E., Sweenie C. H., Smith M., Emery V. C., Hudson M., Tedder R. S., Pounder R. E. Detection of herpesvirus DNA in the large intestine of patients with ulcerative colitis and Crohn's disease using the nested polymerase chain reaction. J Med Virol. 1992 Nov;38(3):183–190. doi: 10.1002/jmv.1890380306. [DOI] [PubMed] [Google Scholar]
- Xu W., Sundqvist V. A., Brytting M., Linde A. Diagnosis of cytomegalovirus infections using polymerase chain reaction, virus isolation and serology. Scand J Infect Dis. 1993;25(3):311–316. doi: 10.3109/00365549309008504. [DOI] [PubMed] [Google Scholar]
- Zurlo J. J., O'Neill D., Polis M. A., Manischewitz J., Yarchoan R., Baseler M., Lane H. C., Masur H. Lack of clinical utility of cytomegalovirus blood and urine cultures in patients with HIV infection. Ann Intern Med. 1993 Jan 1;118(1):12–17. doi: 10.7326/0003-4819-118-1-199301010-00003. [DOI] [PubMed] [Google Scholar]