Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1990 Sep;43(9):719–722. doi: 10.1136/jcp.43.9.719

Use of monoclonal antibody KP1 for identifying normal and neoplastic human mast cells.

H P Horny 1, G Schaumburg-Lever 1, S Bolz 1, M L Geerts 1, E Kaiserling 1
PMCID: PMC502748  PMID: 2212064

Abstract

The monoclonal antibody KP1 (CD68) was used to stain normal and neoplastic monocytes and macrophages in routinely processed, paraffin wax embedded tissue: mast cells also exhibited strong, consistent cytoplasmic immunoreactivity. Light microscopic findings were corroborated by electron microscopical and immunocytochemical findings. The predominant sites of immunoreactivity were the specific intracytoplasmic granules of the mast cells. All mast cell subtypes--that is, normal and reactive mast cells, such as those in lymph nodes exhibiting chronic non-specific lymphadenitis, and malignant or neoplastic mast cells in various types of mastocytosis--reacted with this antibody. This finding is of diagnostic importance, because mast cell proliferation could be mistaken for histiocyte proliferation. It also supports the hypothesis that mast cells derive from the bone marrow.

Full text

PDF
719

Images in this article

720Image
on p.720
720Image
on p.720
720Image
on p.720
721Image
on p.721
721Image
on p.721

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dalton R., Chan L., Batten E., Eridani S. Mast cell leukaemia: evidence for bone marrow origin of the pathological clone. Br J Haematol. 1986 Oct;64(2):397–406. doi: 10.1111/j.1365-2141.1986.tb04133.x. [DOI] [PubMed] [Google Scholar]
  2. FADEM R. S. Tissue mast cells in human bone marrow. Blood. 1951 Jul;6(7):614–630. [PubMed] [Google Scholar]
  3. Gabriel L. C., Escribano L. M., Marie J. P., Zittoun R., Navarro J. L. Peroxidase activity in circulating mast cells in blast crisis of chronic granulocytic leukemia. Comparative studies with basophils and cutaneous mast cells. Am J Clin Pathol. 1986 Aug;86(2):212–219. doi: 10.1093/ajcp/86.2.212. [DOI] [PubMed] [Google Scholar]
  4. Galli S. J. New insights into "the riddle of the mast cells": microenvironmental regulation of mast cell development and phenotypic heterogeneity. Lab Invest. 1990 Jan;62(1):5–33. [PubMed] [Google Scholar]
  5. Horny H. P., Parwaresch M. R., Lennert K. Bone marrow findings in systemic mastocytosis. Hum Pathol. 1985 Aug;16(8):808–814. doi: 10.1016/s0046-8177(85)80252-5. [DOI] [PubMed] [Google Scholar]
  6. Horny H. P., Reimann O., Kaiserling E. Immunoreactivity of normal and neoplastic human tissue mast cells. Am J Clin Pathol. 1988 Mar;89(3):335–340. doi: 10.1093/ajcp/89.3.335. [DOI] [PubMed] [Google Scholar]
  7. Hsu S. M., Raine L., Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577–580. doi: 10.1177/29.4.6166661. [DOI] [PubMed] [Google Scholar]
  8. LEDER L. D. UBER DIE SELEKTIVE FERMENTCYTOCHEMISCHE DARSTELLUNG VON NEUTROPHILEN MYELOISCHEN ZELLEN UND GEWEBSMASTZELLEN IM PARAFFINSCHNITT. Klin Wochenschr. 1964 Jun 1;42:553–553. doi: 10.1007/BF01486688. [DOI] [PubMed] [Google Scholar]
  9. Matsuoka H., Mulrow P. J., Franco-Saenz R., Li C. H. Stimulation of aldosterone production by beta-melanotropin. Nature. 1981 May 14;291(5811):155–156. doi: 10.1038/291155a0. [DOI] [PubMed] [Google Scholar]
  10. Micklem K., Rigney E., Cordell J., Simmons D., Stross P., Turley H., Seed B., Mason D. A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies. Br J Haematol. 1989 Sep;73(1):6–11. doi: 10.1111/j.1365-2141.1989.tb00210.x. [DOI] [PubMed] [Google Scholar]
  11. Parkin J. L., McKenna R. W., Brunning R. D. Philadelphia chromosome-positive blastic leukaemia: ultrastructural and ultracytochemical evidence of basophil and mast cell differentiation. Br J Haematol. 1982 Dec;52(4):663–677. doi: 10.1111/j.1365-2141.1982.tb03943.x. [DOI] [PubMed] [Google Scholar]
  12. Parwaresch M. R., Horny H. P., Lennert K. Tissue mast cells in health and disease. Pathol Res Pract. 1985 Mar;179(4-5):439–461. doi: 10.1016/s0344-0338(85)80184-9. [DOI] [PubMed] [Google Scholar]
  13. Pulford K. A., Rigney E. M., Micklem K. J., Jones M., Stross W. P., Gatter K. C., Mason D. Y. KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections. J Clin Pathol. 1989 Apr;42(4):414–421. doi: 10.1136/jcp.42.4.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ralfkiaer E., Delsol G., O'Connor N. T., Brandtzaeg P., Brousset P., Vejlsgaard G. L., Mason D. Y. Malignant lymphomas of true histiocytic origin. A clinical, histological, immunophenotypic and genotypic study. J Pathol. 1990 Jan;160(1):9–17. doi: 10.1002/path.1711600105. [DOI] [PubMed] [Google Scholar]
  15. Roth J., Bendayan M., Carlemalm E., Villiger W., Garavito M. Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue. J Histochem Cytochem. 1981 May;29(5):663–671. doi: 10.1177/29.5.6166664. [DOI] [PubMed] [Google Scholar]
  16. Schumacher U., Horny H. P., Welsch U. The lectin leucoagglutinin binds specifically to human granulocytes, monocytes and tissue mast cells: further evidence for a common origin of the three cell types. Br J Haematol. 1987 Jul;66(3):405–406. doi: 10.1111/j.1365-2141.1987.tb06931.x. [DOI] [PubMed] [Google Scholar]
  17. Soler J., O'Brien M., de Castro J. T., Miguel J. F., Kearney L., Goldman J. M., Catovsky D. Blast crisis of chronic granulocytic leukemia with mast cell and basophilic precursors. Am J Clin Pathol. 1985 Feb;83(2):254–259. doi: 10.1093/ajcp/83.2.254. [DOI] [PubMed] [Google Scholar]
  18. Travis W. D., Li C. Y., Yam L. T., Bergstralh E. J., Swee R. G. Significance of systemic mast cell disease with associated hematologic disorders. Cancer. 1988 Sep 1;62(5):965–972. doi: 10.1002/1097-0142(19880901)62:5<965::aid-cncr2820620520>3.0.co;2-m. [DOI] [PubMed] [Google Scholar]
  19. Valent P., Ashman L. K., Hinterberger W., Eckersberger F., Majdic O., Lechner K., Bettelheim P. Mast cell typing: demonstration of a distinct hematopoietic cell type and evidence for immunophenotypic relationship to mononuclear phagocytes. Blood. 1989 May 15;73(7):1778–1785. [PubMed] [Google Scholar]
  20. Warnke R. A., Pulford K. A., Pallesen G., Ralfkiaer E., Brown D. C., Gatter K. C., Mason D. Y. Diagnosis of myelomonocytic and macrophage neoplasms in routinely processed tissue biopsies with monoclonal antibody KP1. Am J Pathol. 1989 Dec;135(6):1089–1095. [PMC free article] [PubMed] [Google Scholar]
  21. Werner W., Krause A. R. Die Gewebsmastzellen im menschlichen Knochenmark. Frankf Z Pathol. 1965;74(6):651–658. [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES