Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1992 Nov 1;89(21):10084–10088. doi: 10.1073/pnas.89.21.10084

Retention of habituation in PC12 cells.

L Cheever 1, D E Koshland Jr 1
PMCID: PMC50282  PMID: 1438197

Abstract

The time course of habituation and recovery of neurotransmitter release was measured in neuronally differentiated PC12 cells stimulated with either acetylcholine or ATP. The release of norepinephrine in response to either stimulant declined exponentially with repeated presentation of that stimulant. When the stimulus was withheld, the cells' ability to respond recovered to initial levels with an exponential time course. The rate of response recovery depended on the stimulant used and, in the case of stimulation with acetylcholine, on the number of previous stimuli. After habituation and recovery, or partial recovery, of norepinephrine release, the response to a second series of repetitive stimuli declined more rapidly than in the naive case. This increase in habituation rate was dependent on the number of previous stimuli and, in the case of stimulation with acetylcholine, was stable with time for at least 90 min after stimulation. These phenomena are analogous to characteristics of short- and long-term memories of habituative learning observed in behavioral studies. Kinetic equations based on a putative reversible stimulation-dependent inactivation of the cellular response mechanism were used to analyze the rates of habituation and response recovery.

Full text

PDF
10084

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailey C. H., Chen M. Morphological basis of short-term habituation in Aplysia. J Neurosci. 1988 Jul;8(7):2452–2459. doi: 10.1523/JNEUROSCI.08-07-02452.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Corfas G., Dudai Y. Habituation and dishabituation of a cleaning reflex in normal and mutant Drosophila. J Neurosci. 1989 Jan;9(1):56–62. doi: 10.1523/JNEUROSCI.09-01-00056.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dichter M. A., Tischler A. S., Greene L. A. Nerve growth factor-induced increase in electrical excitability and acetylcholine sensitivity of a rat pheochromocytoma cell line. Nature. 1977 Aug 11;268(5620):501–504. doi: 10.1038/268501a0. [DOI] [PubMed] [Google Scholar]
  4. Goelet P., Castellucci V. F., Schacher S., Kandel E. R. The long and the short of long-term memory--a molecular framework. 1986 Jul 31-Aug 6Nature. 322(6078):419–422. doi: 10.1038/322419a0. [DOI] [PubMed] [Google Scholar]
  5. Gordon J. L. Extracellular ATP: effects, sources and fate. Biochem J. 1986 Jan 15;233(2):309–319. doi: 10.1042/bj2330309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Inoue K., Nakazawa K., Fujimori K., Takanaka A. Extracellular adenosine 5'-triphosphate-evoked norepinephrine secretion not relating to voltage-gated Ca channels in pheochromocytoma PC12 cells. Neurosci Lett. 1989 Dec 4;106(3):294–299. doi: 10.1016/0304-3940(89)90179-1. [DOI] [PubMed] [Google Scholar]
  7. Li Y., Goldbeter A. Frequency specificity in intercellular communication. Influence of patterns of periodic signaling on target cell responsiveness. Biophys J. 1989 Jan;55(1):125–145. doi: 10.1016/S0006-3495(89)82785-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McFadden P. N., Koshland D. E., Jr Habituation in the single cell: diminished secretion of norepinephrine with repetitive depolarization of PC12 cells. Proc Natl Acad Sci U S A. 1990 Mar;87(5):2031–2035. doi: 10.1073/pnas.87.5.2031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McFadden P. N., Koshland D. E., Jr Parallel pathways for habituation in repetitively stimulated PC12 cells. Neuron. 1990 Apr;4(4):615–621. doi: 10.1016/0896-6273(90)90119-z. [DOI] [PubMed] [Google Scholar]
  10. Pinsker H., Kupfermann I., Castellucci V., Kandel E. Habituation and dishabituation of the gill-withdrawal reflex in Aplysia. Science. 1970 Mar 27;167(3926):1740–1742. doi: 10.1126/science.167.3926.1740. [DOI] [PubMed] [Google Scholar]
  11. Thompson R. F., Spencer W. A. Habituation: a model phenomenon for the study of neuronal substrates of behavior. Psychol Rev. 1966 Jan;73(1):16–43. doi: 10.1037/h0022681. [DOI] [PubMed] [Google Scholar]
  12. Vicentini L. M., Ambrosini A., Di Virgilio F., Meldolesi J., Pozzan T. Activation of muscarinic receptors in PC12 cells. Correlation between cytosolic Ca2+ rise and phosphoinositide hydrolysis. Biochem J. 1986 Mar 15;234(3):555–562. doi: 10.1042/bj2340555. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES