Abstract
Hepatitis C virus (HCV) replication is reported in both tumour and non-tumour tissue in a case of hepatocellular carcinoma. Viral replication was established by showing the presence of minus strand HCV RNA by PCR amplification, after excluding residual reverse transcriptase activity of Taq polymerase. No minus strand was found in serum derived virion RNA. PCR amplified products from both tumour and non-tumour parenchyma were sequenced in the 5' non-coding region and shown to be identical. The genotype of this Indonesian patient was found to be 1b (or II), the most prevalent type in the Far East.
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- Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
- Chou W. H., Yoneyama T., Takeuchi K., Harada H., Saito I., Miyamura T. Discrimination of hepatitis C virus in liver tissues from different patients with hepatocellular carcinomas by direct nucleotide sequencing of amplified cDNA of the viral genome. J Clin Microbiol. 1991 Dec;29(12):2860–2864. doi: 10.1128/jcm.29.12.2860-2864.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Colombo M., Kuo G., Choo Q. L., Donato M. F., Del Ninno E., Tommasini M. A., Dioguardi N., Houghton M. Prevalence of antibodies to hepatitis C virus in Italian patients with hepatocellular carcinoma. Lancet. 1989 Oct 28;2(8670):1006–1008. doi: 10.1016/s0140-6736(89)91016-7. [DOI] [PubMed] [Google Scholar]
- Dourakis S., Brown J., Kumar U., Karayiannis P., Kernoff P., Chiba J., Ohba H., Miyamura T., Saito I., Monjardino J. Serological response and detection of viraemia in acute hepatitis C virus infection. J Hepatol. 1992 Mar;14(2-3):370–376. doi: 10.1016/0168-8278(92)90185-r. [DOI] [PubMed] [Google Scholar]
- Gerber M. A., Shieh Y. S., Shim K. S., Thung S. N., Demetris A. J., Schwartz M., Akyol G., Dash S. Detection of replicative hepatitis C virus sequences in hepatocellular carcinoma. Am J Pathol. 1992 Dec;141(6):1271–1277. [PMC free article] [PubMed] [Google Scholar]
- Kumar U., Brown J., Monjardino J., Thomas H. C. Sequence variation in the large envelope glycoprotein (E2/NS1) of hepatitis C virus during chronic infection. J Infect Dis. 1993 Mar;167(3):726–730. doi: 10.1093/infdis/167.3.726. [DOI] [PubMed] [Google Scholar]
- Shimizu Y. K., Iwamoto A., Hijikata M., Purcell R. H., Yoshikura H. Evidence for in vitro replication of hepatitis C virus genome in a human T-cell line. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5477–5481. doi: 10.1073/pnas.89.12.5477. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simmonds P., McOmish F., Yap P. L., Chan S. W., Lin C. K., Dusheiko G., Saeed A. A., Holmes E. C. Sequence variability in the 5' non-coding region of hepatitis C virus: identification of a new virus type and restrictions on sequence diversity. J Gen Virol. 1993 Apr;74(Pt 4):661–668. doi: 10.1099/0022-1317-74-4-661. [DOI] [PubMed] [Google Scholar]
- Takeda S., Shibata M., Morishima T., Harada A., Nakao A., Takagi H., Nagai Y. Hepatitis C virus infection in hepatocellular carcinoma. Detection of plus-strand and minus-strand viral RNA. Cancer. 1992 Nov 1;70(9):2255–2259. doi: 10.1002/1097-0142(19921101)70:9<2255::aid-cncr2820700907>3.0.co;2-n. [DOI] [PubMed] [Google Scholar]
- van Doorn L. J., Kleter B., Stuyver L., Maertens G., Brouwer H., Schalm S., Heijtink R., Quint W. Analysis of hepatitis C virus genotypes by a line probe assay and correlation with antibody profiles. J Hepatol. 1994 Jul;21(1):122–129. doi: 10.1016/s0168-8278(94)80148-7. [DOI] [PubMed] [Google Scholar]