Abstract
Isolates of Campylobacter jejuni, C coli, C fetus and C laridis were tested for agglutination reactions with a panel of five lectins: Arachis hypogaea, Bauhinia purpurea, Solanum tuberosum, Triticum vulgaris and Wisteria floribunda. Twenty three patterns of agglutination (lectin types) were recorded among 376 isolates. Patterns were consistent and reproducible. Only 4.5% of isolates were untypable because of autoagglutination. Some lectin types were found exclusively or predominantly in a species, but others were shared between species. Forty two per cent of C jejuni and 35% of C coli isolates belonged to lectin type 4. There was no apparent correlation between lectin type and serotype; different lectin types were found among strains of single Penner and Lior serotypes. Lectin typing is a simple and economical procedure suitable for use in non-specialist laboratories, either as an adjunct to serogrouping or, after further development, as a sole typing scheme.
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Selected References
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- Beer W., Adam M., Seltmann G. Monosaccharide composition of lipopolysaccharides from Campylobacter jejuni and Campylobacter coli. J Basic Microbiol. 1986;26(4):201–204. doi: 10.1002/jobm.3620260405. [DOI] [PubMed] [Google Scholar]
- Cole H. B., Ezzell J. W., Jr, Keller K. F., Doyle R. J. Differentiation of Bacillus anthracis and other Bacillus species by lectins. J Clin Microbiol. 1984 Jan;19(1):48–53. doi: 10.1128/jcm.19.1.48-53.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davidson S. K., Keller K. F., Doyle R. J. Differentiation of coagulase-positive and coagulase-negative staphylococci by lectins and plant agglutinins. J Clin Microbiol. 1982 Apr;15(4):547–553. doi: 10.1128/jcm.15.4.547-553.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Doyle R. J., Nedjat-Haiem F., Miller R. D., Keller K. F. Interaction between plant agglutinins and Legionella species. J Clin Microbiol. 1982 May;15(5):973–975. doi: 10.1128/jcm.15.5.973-975.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Frasch C. E. Role of lipopolysaccharide in wheat germ agglutinin-mediated agglutination of Neisseria meningitidis and Neisseria gonorrhoeae. J Clin Microbiol. 1980 Oct;12(4):498–501. doi: 10.1128/jcm.12.4.498-501.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gallagher J. T. Carbohydrate-binding properties of lectins: a possible approach to lectin nomenclature and classification. Review. Biosci Rep. 1984 Aug;4(8):621–632. doi: 10.1007/BF01121015. [DOI] [PubMed] [Google Scholar]
- Lior H., Woodward D. L., Edgar J. A., Laroche L. J., Gill P. Serotyping of Campylobacter jejuni by slide agglutination based on heat-labile antigenic factors. J Clin Microbiol. 1982 May;15(5):761–768. doi: 10.1128/jcm.15.5.761-768.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Naess V., Hofstad T. Chemical composition and biological activity of lipopolysaccharides prepared from type strains of Campylobacter jejuni and Campolybacter coli. Acta Pathol Microbiol Immunol Scand B. 1984 Aug;92(4):217–222. doi: 10.1111/j.1699-0463.1984.tb02824.x. [DOI] [PubMed] [Google Scholar]
- Sharon N., Lis H. Lectins: cell-agglutinating and sugar-specific proteins. Science. 1972 Sep 15;177(4053):949–959. doi: 10.1126/science.177.4053.949. [DOI] [PubMed] [Google Scholar]
- Wong K. H., Skelton S. K., Feeley J. C. Interaction of Campylobacter jejuni and Campylobacter coli with lectins and blood group antibodies. J Clin Microbiol. 1985 Jul;22(1):134–135. doi: 10.1128/jcm.22.1.134-135.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wong K. H., Skelton S. K., Feeley J. C. Strain characterization and grouping of Campylobacter jejuni and Campylobacter coli by interaction with lectins. J Clin Microbiol. 1986 Mar;23(3):407–410. doi: 10.1128/jcm.23.3.407-410.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
