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. 1995 Oct;48(10):950–954. doi: 10.1136/jcp.48.10.950

Immunohistochemical identification of tumours of adipocytic differentiation using an antibody to aP2 protein.

J H Bennett 1, S Shousha 1, B Puddle 1, N A Athanasou 1
PMCID: PMC502954  PMID: 8537497

Abstract

AIM--To determine whether aP2 expression is a useful diagnostic marker in soft tissue tumour pathology. METHODS--A polyclonal antibody to aP2 was used to investigate the immunohistochemical expression of this protein in benign and malignant tumours of adipocytic differentiation and a wide variety of other neoplasms. RESULTS--aP2 was only expressed by lipoblasts (in all types of liposarcoma and in lipoblastomatosis) and by brown fat cells (in both hibernomas and normal periadrenal fetal fat). Other benign adipose tissue tumours and malignant connective tissue or epithelial tumours were distinguished from liposarcoma by the absence of staining for aP2. CONCLUSION--Identification of lipoblasts using markers of aP2 expression is of value in the differential diagnosis of benign and malignant adipose tissue tumours and in distinguishing liposarcomas from other malignant mesenchymal and epithelial neoplasms, some of which contain cells that morphologically resemble lipoblasts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Azzopardi J. G., Iocco J., Salm R. Pleomorphic lipoma: a tumour simulating liposarcoma. Histopathology. 1983 Jul;7(4):511–523. doi: 10.1111/j.1365-2559.1983.tb02264.x. [DOI] [PubMed] [Google Scholar]
  2. Baxa C. A., Sha R. S., Buelt M. K., Smith A. J., Matarese V., Chinander L. L., Boundy K. L., Bernlohr D. A. Human adipocyte lipid-binding protein: purification of the protein and cloning of its complementary DNA. Biochemistry. 1989 Oct 31;28(22):8683–8690. doi: 10.1021/bi00448a003. [DOI] [PubMed] [Google Scholar]
  3. Beresford J. N., Bennett J. H., Devlin C., Leboy P. S., Owen M. E. Evidence for an inverse relationship between the differentiation of adipocytic and osteogenic cells in rat marrow stromal cell cultures. J Cell Sci. 1992 Jun;102(Pt 2):341–351. doi: 10.1242/jcs.102.2.341. [DOI] [PubMed] [Google Scholar]
  4. Bernlohr D. A., Angus C. W., Lane M. D., Bolanowski M. A., Kelly T. J., Jr Expression of specific mRNAs during adipose differentiation: identification of an mRNA encoding a homologue of myelin P2 protein. Proc Natl Acad Sci U S A. 1984 Sep;81(17):5468–5472. doi: 10.1073/pnas.81.17.5468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bernlohr D. A., Doering T. L., Kelly T. J., Jr, Lane M. D. Tissue specific expression of p422 protein, a putative lipid carrier, in mouse adipocytes. Biochem Biophys Res Commun. 1985 Oct 30;132(2):850–855. doi: 10.1016/0006-291x(85)91209-4. [DOI] [PubMed] [Google Scholar]
  6. Cattoretti G., Pileri S., Parravicini C., Becker M. H., Poggi S., Bifulco C., Key G., D'Amato L., Sabattini E., Feudale E. Antigen unmasking on formalin-fixed, paraffin-embedded tissue sections. J Pathol. 1993 Oct;171(2):83–98. doi: 10.1002/path.1711710205. [DOI] [PubMed] [Google Scholar]
  7. Dias P., Parham D. M., Shapiro D. N., Webber B. L., Houghton P. J. Myogenic regulatory protein (MyoD1) expression in childhood solid tumors: diagnostic utility in rhabdomyosarcoma. Am J Pathol. 1990 Dec;137(6):1283–1291. [PMC free article] [PubMed] [Google Scholar]
  8. Dieckmann U., Marrow P., Law R. Evolutionary cycling in predator-prey interactions: population dynamics and the red queen. J Theor Biol. 1995 Sep 7;176(1):91–102. doi: 10.1006/jtbi.1995.0179. [DOI] [PubMed] [Google Scholar]
  9. Grigoriadis A. E., Heersche J. N., Aubin J. E. Differentiation of muscle, fat, cartilage, and bone from progenitor cells present in a bone-derived clonal cell population: effect of dexamethasone. J Cell Biol. 1988 Jun;106(6):2139–2151. doi: 10.1083/jcb.106.6.2139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nakajima T., Watanabe S., Sato Y., Kameya T., Hirota T., Shimosato Y. An immunoperoxidase study of S-100 protein distribution in normal and neoplastic tissues. Am J Surg Pathol. 1982 Dec;6(8):715–727. doi: 10.1097/00000478-198212000-00003. [DOI] [PubMed] [Google Scholar]
  11. Poissonnet C. M., LaVelle M., Burdi A. R. Growth and development of adipose tissue. J Pediatr. 1988 Jul;113(1 Pt 1):1–9. doi: 10.1016/s0022-3476(88)80520-1. [DOI] [PubMed] [Google Scholar]
  12. Shmookler B. M., Enzinger F. M. Pleomorphic lipoma: a benign tumor simulating liposarcoma. A clinicopathologic analysis of 48 cases. Cancer. 1981 Jan 1;47(1):126–133. doi: 10.1002/1097-0142(19810101)47:1<126::aid-cncr2820470121>3.0.co;2-k. [DOI] [PubMed] [Google Scholar]
  13. Spiegelman B. M., Choy L., Hotamisligil G. S., Graves R. A., Tontonoz P. Regulation of adipocyte gene expression in differentiation and syndromes of obesity/diabetes. J Biol Chem. 1993 Apr 5;268(10):6823–6826. [PubMed] [Google Scholar]
  14. Taylor S. M., Jones P. A. Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine. Cell. 1979 Aug;17(4):771–779. doi: 10.1016/0092-8674(79)90317-9. [DOI] [PubMed] [Google Scholar]

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