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. 2016 Mar 31;7(17):23056–23071. doi: 10.18632/oncotarget.8521

Figure 3. BMAL1 controls HIF-1 activity without affecting HIF-α-TAD function.

Figure 3

A. Evaluation of BMAL1 and RORα expression by Western blot in NP cells stably transduced with lentivirus expressing BMAL1 shRNA. B., C. Densitometric analysis of multiple blots shown in (A) demonstrated decreased BMAL1 (B) and RORα (C) expression in BMAL1-silenced cells under both normoxia and hypoxia. D. HRE activity of BMAL1-silenced NP cells is significantly lower than cells transduced with control shRNA. E.-G. Evaluation of BMAL1 control of HIF-α-TAD function. BMAL1 overexpression had no effects on HIF-1α-C-TAD (E), HIF-1α-N-TAD (F), as well as HIF-2α-TAD (G) regardless of oxygen tension. Data is represented as mean ± SE, n= 3, * p<0.05.