Figure 5. Expression of RASA1 wild type (WT), but not Y472H and L481F mutants, suppresses anchorage-independent growth in vitro and tumor growth in vivo.

Wild type (WT), Y472H, or L481F mutant form of RASA1 was expressed in UACC257 (A) and WM983C (B and C). (A and B) Ectopic expression was confirmed by immunoblotting with anti-RASA1 antibody (top). Graph shows the mean (+/− SD) number of colonies grown on soft agar on day 21 counted per well (Bottom). (C) Subcutaneous tumors generated with WM983C cells (top) and tumor weights (bottom) on day 29 for each group. Statistical analysis was performed using one-way ANOVA with Tukey's multiple comparison adjustment. Data shown graphically as mean +/− SD.