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. 1992 Nov 1;89(21):10159–10163. doi: 10.1073/pnas.89.21.10159

Cell division arrest induced by phorbol ester in CHO cells overexpressing protein kinase C-delta subspecies.

T Watanabe 1, Y Ono 1, Y Taniyama 1, K Hazama 1, K Igarashi 1, K Ogita 1, U Kikkawa 1, Y Nishizuka 1
PMCID: PMC50297  PMID: 1438205

Abstract

Several lines of CHO cells stably overexpressing protein kinase C (PKC) subspecies to various extents were established by the DNA-mediated transfer. Upon treatment with phorbol 12-myristate 13-acetate, the growth of the cells expressing the PKC-delta subspecies was markedly inhibited, whereas cell lines expressing PKC-alpha, PKC-beta II, and PKC-zeta subspecies were not significantly affected. Flow cytometric analysis indicated that all cell lines overexpressing PKC-delta subspecies accumulated in G2/M phase in response to phorbol 12-myristate 13-acetate. In these arrested cells, dikaryons were predominant, implying that phorbol ester-induced inhibition of cell division is specific to telophase. These results suggest PKC-delta subspecies may play a role in the normal cell cycle progression.

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