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. 1992 Nov 1;89(21):10164–10168. doi: 10.1073/pnas.89.21.10164

Human monoclonal Fab fragments derived from a combinatorial library bind to respiratory syncytial virus F glycoprotein and neutralize infectivity.

C F Barbas 3rd 1, J E Crowe Jr 1, D Cababa 1, T M Jones 1, S L Zebedee 1, B R Murphy 1, R M Chanock 1, D R Burton 1
PMCID: PMC50298  PMID: 1279672

Abstract

Respiratory syncytial virus (RSV) is the most important cause, throughout the world, of severe viral lower respiratory tract illness in young children. Antibodies are known to mediate resistance to RSV infection and illness. We have isolated a number of human monoclonal Fab fragments to RSV F glycoprotein from a combinatorial antibody library expressed on the surface of phage. One of these neutralized a wide range of virus isolates, 10 subgroup A and 9 subgroup B isolates, with a titer (60% neutralization) of approximately 0.1-1.0 micrograms/ml. Another Fab neutralized diverse isolates at a concentration somewhat higher. These human Fab fragments show great promise for use in the prophylaxis or therapy of serious RSV lower respiratory tract disease. For intramuscular or intravenous administration, whole antibodies will be required, whereas for aerosol application, F(ab')2 or Fab fragments may suffice.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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