Abstract
AIMS--To describe the ultrastructural changes that occur in human antral mucosa following direct application of aspirin in volunteers without Helicobacter pylori infection. METHODS--Ten healthy male volunteers without H pylori infection underwent three consecutive endoscopies (at zero, one and five hours). At the first endoscopy, two biopsy specimens were obtained (one for histology and the other for electron microscopy (EM)). At subsequent endoscopies, a single biopsy specimen was obtained for EM. A 50 ml solution of aspirin (concentration 3 mg/ml) was applied to the antral mucosa at the first endoscopy in five subjects; the other five subjects received 50 ml distilled water (placebo). RESULTS--The ultrastructural appearance of the first biopsy specimen in all subjects and subsequent biopsy specimens in the placebo treated subjects was normal. The aspirin treated group had evidence of intercellular oedema, widening of capillary fenestrae, rupturing of apical membranes, and dilatation of endoplasmic reticulum and mitochondria after one hour; these changes were more marked at five hours. Tight junctions were maintained. CONCLUSION--This is the first study to describe the early ultrastructural changes in antral mucosa induced by aspirin in subjects without H pylori infection.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Haas R., Parker W. D., Jr, Stumpf D., Eguren L. A. Salicylate-induced loose coupling: protonmotive force measurements. Biochem Pharmacol. 1985 Mar 15;34(6):900–902. doi: 10.1016/0006-2952(85)90774-9. [DOI] [PubMed] [Google Scholar]
- Kauffman G. Aspirin-induced gastric mucosal injury: lessons learned from animal models. Gastroenterology. 1989 Feb;96(2 Pt 2 Suppl):606–614. doi: 10.1016/s0016-5085(89)80056-3. [DOI] [PubMed] [Google Scholar]
- Kazi J. L., Sinniah R., Zaman V., Ng M. L., Jafarey N. A., Alam S. M., Zuberi S. J., Kazi A. M. Ultrastructural study of Helicobacter pylori-associated gastritis. J Pathol. 1990 May;161(1):65–70. doi: 10.1002/path.1711610111. [DOI] [PubMed] [Google Scholar]
- Meschter C. L., Gilbert M., Krook L., Maylin G., Corradino R. The effects of phenylbutazone on the intestinal mucosa of the horse: a morphological, ultrastructural and biochemical study. Equine Vet J. 1990 Jul;22(4):255–263. doi: 10.1111/j.2042-3306.1990.tb04264.x. [DOI] [PubMed] [Google Scholar]
- Rainsford K. D., Willis C. M., Walker S. A., Robins P. G. Electron microscopic observations comparing the gastric mucosal damage induced in rats and pigs by benoxaprofen and aspirin, reflecting their differing actions as prostaglandin-synthesis-inhibitors. Br J Exp Pathol. 1982 Feb;63(1):25–34. [PMC free article] [PubMed] [Google Scholar]
- Robins P. G. Ultrastructural observations on the pathogenesis of aspirin-induced gastric erosions. Br J Exp Pathol. 1980 Oct;61(5):497–504. [PMC free article] [PubMed] [Google Scholar]
- Tricottet V., Bruneval P., Vire O., Camilleri J. P., Bloch F., Bonte N., Roge J. Campylobacter-like organisms and surface epithelium abnormalities in active, chronic gastritis in humans: an ultrastructural study. Ultrastruct Pathol. 1986;10(2):113–122. doi: 10.3109/01913128609014587. [DOI] [PubMed] [Google Scholar]