Figure 8. Acute stimulation of the CSDn alters VA1d responses via cholinergic transmission.

(A) VA1d odor responses to methyl laurate were sampled every 80 s before, during, and after stimulation of the CSDn with Chrimson. Stimulation consisted of a 10 Hz sine wave that was interrupted briefly to sample VA1d response. (B) Chronic stimulation of the CSDn did not significantly alter VA1d responses either in saline (n = 7, p=0.77) or methysergide (n = 6, p=0.78). (C) A mean PSTH of VA1d odor responses with (red trace) and without (black trace) simulatneous CSDn activation with Chrimson (40 ms). (D) A raster showing an increase in the odor respnse of VA1d PNs during brief CSDn stimulation. Methyl laurate was presented undiluted. (E) In saline, PN firing over the entire duration of the odor response was unchanged (left, n = 7, p=0.84) but during the 40 ms of CSDn stimulation, VA1d responses increased (right,, n = 7, p=0.046). (F) In methysergide, PN firing over the entire duration of the odor response was also unchanged (left, n = 6, p=0.16). During the 40 ms of CSDn stimulation, VA1d responses increased (right, n = 6, p=0.008).

DOI: http://dx.doi.org/10.7554/eLife.16836.016

Figure 8—figure supplement 1. Acute stimulation of the CSDn does not modulate DA1 odor respones.

(A) A PSTH of DA1 odor responses to cVA. Responses were sampled at 80 s intervals and the CSDn was stimulated via Chrimson with 40 ms pulses of red light. The timing of the light pulses was set to occur roughly during th peak of the odor responses. The delay seen between odor onset and odor response is due to the movement of odors through the olfactometer and to the preparation. (B) Left, DA1 odor respones were not significantly changed over the duration of the odor response, n = 6, p=0.280, paired t-test. This analysis window was 500 ms starting from the onset of the response seen in the PSTH. Right, DA1 responses were also not statistically different during the brief 40 ms of CSDn stimulation, n = 6, p=0.971. (C) PSTH of DA1 responses with co-activation of CSDn stimulation in the presence of methysergide. DA1 responses were not significantly altered during the entire stimulus window (left, n = 6, p=0.287) or the brief period of CSDn stimulation (right, n = 6, p=0.141).

DOI: http://dx.doi.org/10.7554/eLife.16836.017