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Nature Communications logoLink to Nature Communications
. 2016 Sep 19;7:12875. doi: 10.1038/ncomms12875

Dysferlin-mediated phosphatidylserine sorting engages macrophages in sarcolemma repair

Volker Middel 1, Lu Zhou 2,3, Masanari Takamiya 1, Tanja Beil 1, Maryam Shahid 1, Urmas Roostalu 4, Clemens Grabher 1, Sepand Rastegar 1, Markus Reischl 5, Gerd Ulrich Nienhaus 1,2,3,6, Uwe Strähle 1,a
PMCID: PMC5031802  PMID: 27641898

Abstract

Failure to repair the sarcolemma leads to muscle cell death, depletion of stem cells and myopathy. Hence, membrane lesions are instantly sealed by a repair patch consisting of lipids and proteins. It has remained elusive how this patch is removed to restore cell membrane integrity. Here we examine sarcolemmal repair in live zebrafish embryos by real-time imaging. Macrophages remove the patch. Phosphatidylserine (PS), an ‘eat-me' signal for macrophages, is rapidly sorted from adjacent sarcolemma to the repair patch in a Dysferlin (Dysf) dependent process in zebrafish and human cells. A previously unrecognized arginine-rich motif in Dysf is crucial for PS accumulation. It carries mutations in patients presenting with limb-girdle muscular dystrophy 2B. This underscores the relevance of this sequence and uncovers a novel pathophysiological mechanism underlying this class of myopathies. Our data show that membrane repair is a multi-tiered process involving immediate, cell-intrinsic mechanisms as well as myofiber/macrophage interactions.

Inline graphicSarcolemma lesions are sealed by a repair patch of lipids and proteins that prevents cell death and myopathy. Here the authors show that the "eat-me" signal phosphatidylserine is sorted from adjacent sarcolemma to the repair patch in a Dysferlin dependent process in zebrafish and human cells.

Skeletal muscle cells are prone to plasma membrane lesions under physiological levels of mechanical stress. To prevent cell death and to avoid muscle regeneration with concomitant depletion of stem cell pools, lesions are rapidly sealed by a repair patch consisting of proteins and lipids1,2. The transmembrane protein Dysf plays a key role in restoring cell integrity. Humans with mutations in the DYSF gene acquire limb-girdle muscular dystrophy type 2B (LGMD2B), Miyoshi myopathy or distal myopathy with anterior tibialis onset3,4. Dysf binds phosphatidylserine (PS) in a Ca2+-dependent manner through its C2-domains5. Mini-Dysf generated by Ca2+-dependent calpain proteases and comprising the last two C2-domains of the full length mammalian protein appears to be the isoform of Dysf active in membrane repair6. In zebrafish, deletion of the C2 domains generated an isoform that is still able to accumulate at the site of lesion7. How Dysf precisely acts in membrane repair has remained elusive. Other players in membrane repair are annexins (AnxAs), which associate into multimeric complexes in a Ca2+-dependent manner at the lesion8,9,10. These complexes are built by homo and heteromeric interactions of AnxAs and include also Dysf and possibly other repair proteins and lipids7,8,9,10. The distinct temporal order of proteins' arrival in the repair patch suggests that a specific structure of the protein-lipid matrix is required for its function7. Knock-down of Dysf or AnxA6 translation caused a malformed repair patch and double morphants even showed leakage of cytoplasmic components into the extracellular environment7. The origin of the membrane material forming the repair patch is unclear. Lysosomes as well as endosomes were suggested to contribute to membrane repair in mammalian cell systems11,12. However, none of the tested intracellular vesicles marked by Laptm4a, Lamp1, Lamp2, Rab1a, Rab5a, Rab6a, Rab7 and Rab27a contribute significantly to repair patch formation in zebrafish myofibers7. Although mechanisms like membrane shedding, exocytosis and endocytosis were discussed, a still unresolved issue is how the repair patch is removed to restore the plasma membrane2.

We have employed live fluorescence imaging including single-molecule based techniques to study membrane repair in muscle cells in real time in zebrafish embryos and human cells. We provide evidence that macrophages remove the repair patch from damaged cells involving selective enrichment of PS at the lesion. Strikingly, Dysf mediates PS transport from adjacent sarcolemma to the repair patch through a five amino acid (AA)-motif close to its transmembrane (TM) domain, harbouring a point mutation in certain LGMD2B patients.

Results

Macrophages remove repair patch

To assess up to which size a sarcolemmal lesion can still be repaired, we damaged the sarcolemma of single myofibers in the somitic musculature co-expressing plasmids encoding Ca2+ sensing GCaMP5A (ref. 13) together with mOrange-tagged AnnexinA2a (AnxA2a-mO)7. After a transient increase of Ca2+ ions at the lesion, a patch rapidly formed. All fibres with lesions ≤4 μm survived membrane damage whereas lesions ⩾4 μm were associated with cell death (Supplementary Fig. 1a–g, Supplementary Movie 1, 2).

Frequently, we observed a motile cell appearing at the repair patch. We hypothesized neutrophils or macrophages were attracted to these sites and utilized Tg(mpeg1:GFP) and Tg(lysC:dsRed) marking macrophages14 and neutrophils15, respectively. The membranes of AnxA2a-mO expressing myofibers of transgenic fish were injured and leucocyte migration was monitored. Macrophages were recruited to injured myofibers (Fig. 1a–c, Supplementary Movie 3) in 50% of the cases within 16.4±11.6 min (n=92) after injury. In long-term (16–20 h) time-lapse experiments, 92% of the damaged myofibers had been visited by macrophages (n=143) (Supplementary Fig. 2a–c,g; Supplementary Movie 4). While myofibers with small lesions (≤4 μm) attracted on average 1.29±0.2 macrophages (n=53), about twice as many macrophages (2.5±1.4, n=87) were encountered at cells with lesions ⩾4 μm. Macrophages were observed to ingest parts of the repair patch in surviving cells with lesion smaller than 4 μm (Fig. 1a,c). In contrast, macrophages phagocytosed entire myofibers with lesions >4 μm (Supplementary Fig. 2a–c). Neutrophils were detected only infrequently (7%, n=126) at laser induced membrane lesions and did not participate in phagocytosis of dead myofibers. In contrast, sterility-compromised stabwounds inflicted by insertion of a glass needle into the somitic musculature attracted neutrophils in all cases (n=23) similar to tailfin cuts (n=12; Supplementary Fig. 2h).

Figure 1. Macrophages remove repair patch.

Figure 1

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(a–c) Repair patch (AnxA2a-mO, a, white arrow) and macrophages (mpeg1:GFP, b, white arrows) before (Initial), 240 s and 1,500 s after wounding. A macrophage ingests parts of repair patch (green arrows). (c) Merged views of a,b. Note the bleed-through of AnxA2a-mO into the GFP channel (b). (d) Repair patch removal in control (left, n=37) and macrophage-depleted embryos (KD, right, n=25, Fisher's exact test P<10−8) over 20 h. (e) Crispr/Cas9 knock-out of irf8 impairs repair patch removal. In controls, the repair patch was removed in 32% of injured myofibers (n=19) within one hour after injury. In the irf8-KO embryos the patch was present in all cases of injured myofibers examined (n=15). This suggests that morpholino triple knock-down is inefficient and the same effect on macrophages can be achieved by elimination of irf8 at the stage analysed. Significance was checked with Fishers exact test at P<0.05 (P=0.023895). Scale bar, 4 μm.

To enquire if macrophages are crucial for repair patch removal, we employed an established morpholino knock-down strategy16,17,18. By triple injection of morpholinos directed against gcsfr, pu1 and irf8 mRNAs, we eliminated Tg(mpeg1:GFP) expressing macrophages. In controls (Fig. 1d, n=37), the repair patch was removed in 62% of the damaged cells, whereas, in all morphants (Fig. 1d, n=25, P<10−8), the patch persisted beyond the observation time (20 h). Myofibers damaged beyond repair were not phagocytosed in the morphants (Supplementary Fig. 2d–g, n=20), whereas 92% were removed within 20 h in controls (Supplementary Fig. 2a–c, g, n=12; P<10−8). We obtained the same results when we analysed a Crispr/Cas9 generated loss-of-function mutation in irf8 (Fig. 1e) underscoring that the observed morpholino effects were specific. Taken together, these findings strongly suggest a crucial and novel role of macrophages for selectively removing the repair patch at membrane lesions.

Fast and selective PS accumulation

Exposure of PS to the extracellular space can trigger phagocytosis by macrophages19. To investigate, whether PS accumulates in the repair patch, we co-expressed the stereo-specific and Ca2+-independent PS sensor LactAdherinC2:GFP (LactC2:GFP)20 together with AnxA2a-mO. The PS sensor accumulated earlier than AnxA2a-mO (Fig. 2a, Supplementary Fig. 3a; Supplementary Movie 5). Dysf fused to mOrange (Dysf–mO)7 accumulated at the same pace as LactC2:GFP (Fig. 2b, Supplementary Fig. 3b) or TopFluor-PS (ref. 21) at membrane lesions (Supplementary Fig. 3c). Clearly, PS is one key component of the early repair patch.

Figure 2. Phosphatidylserine (PS) is sorted to the repair patch.

Figure 2

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(a–h) Real-time analysis of enrichment of PS (LactC2:GFP (a–c)), PI(4,5)P2 (GFP-2 × PH(PLCδ) (e)), PIP3 (pEGFP::2FYVE–GFP (f)), PI(3,4)P2 (AKT-PH:EGFP (g)) and cholesterol (BODIPY-cholesterol (h)), relative to AnxA2a-mO (a), Dysf–mO (b) and membrane markers CAAX-mCherry (c), Lyn-tailed mCherry (d). The reporter fluorescence is expressed as percentage (mean±s.d.) relative to the level before injury. (i–m) PS is presented on the extracellular side of the repair patch. (i–l) Extracellularly supplied secA5-YFP was enriched at membrane on lesioning (i–k, arrows). secA5-YFP expressing myofibers are outside of the field of view. (l) Kinetics of secA5-YFP at the repair patch. (m) Macrophage scanning-time (+/− s.d.) of myofibers expressing secreted secA5-YFP (yellow, n=19), AnxA2a-mO (dark red, n=22) or Dysf–mO (light red, n=23; Student t-test P<0.001) Scale bar, 4 μm.

We next analysed the selectivity of PS accumulation. Membrane tethered CAAX-mCherry22 accumulated more slowly (Fig. 2c, Supplementary Fig. 3d) compared with LactC2:GFP (Fig. 2c). Lyn-tailed mCherry23, another membrane marker, showed no accumulation at the lesion (Fig. 2d, Supplementary Fig. 3e). We also tested lipid sensors recognizing phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2, GFP-2 × PH(PLCδ))24, phosphatidylinositol (3,4,5)-trisphosphate (PIP3; pEGFP::2FYVE–GFP)25, phosphatidylinositol 3,4-bisphosphate (PI(3,4)P2, AKT-PH:EGFP)25,26 and a fluorescently tagged cholesterol (BODIPY-cholesterol)27. Except for cholesterol (Fig. 2h, Supplementary Fig. 3i), none of the lipid markers accumulated at levels comparable to PS (Fig. 2e–g, Supplementary Fig. 3f–h).

To assess whether PS is presented to macrophages at the repair patch, we expressed the secreted PS sensor secA5-YFP (ref. 28) and injured non-expressing myofibers (Fig. 2i–l). Hence, secA5-YFP was exclusively supplied to the lesion patch from the extracellular space (Fig. 2j,k). secA5-YFP was enriched at the lesion (Fig. 2l, Supplementary Movie 6) demonstrating that PS is present at the extracellular surface of the lesion patch.

We reasoned that secA5-YFP masks PS at the lesion patch and thereby delays phagocytosis. Indeed, phagocytosis was delayed in embryos expressing secA5-YFP (n=19; 349±241 min) in comparison with Dysf–mO (n=23; 108±104 min; P<0.00001) or AnxA2a-mO (n=22; 124±79 min; P<0.00001) expressing fibres (Fig. 2m). Taken together, PS rapidly and selectively accumulates in the lesion patch, is presented to the outside, and masking of PS delays phagocytosis. All these observations clearly support the notion that PS marks the repair patch for phagocytosis by macrophages.

Dysf is required for PS accumulation

Since Dysf fragments and LactC2:GFP accumulated at similar speeds, we tested whether Dysf plays an active role in PS accumulation. We inflicted membrane wounds in myofibers expressing LactC2:GFP in combination with morpholino (MO) knock-down of endogenous Dysf7. Injecting a 5 bp mismatch contr-MO caused normal PS accumulation (Fig. 3a,c). Injection of dysf–MO, however, abolished PS recruitment (Fig. 3b,c, Supplementary Fig. 4a). Co-injection of dysf–MO together with mOrange1–DysfC7 restored accumulation of PS (Fig. 3c, Supplementary Fig. 4a). AnxA6 is required for the formation of a tight lesion patch7. In anxa6 morphants, PS accumulation was indistinguishable from that of controls (Fig. 3d, Supplementary Fig. 4b). Furthermore, dysf-mutant embryos, in contrast to wild-type siblings showed significantly reduced PS accumulation (Supplementary Fig. 4c–f).

Figure 3. Five amino acid motif in Dysf is required for PS accumulation.

Figure 3

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(a,b) Before wounding, LactC2:GFP (green) localized to the sarcolemma (sc; a) and the Z-line (z; a) both in control (a) and knock-down (dysf-KD; b) myofibers. On injury, LactC2:GFP accumulated in controls (a, arrow) but not in dysf-KD myofibers (b, arrow). (c) Kinetics of LactC2:GFP accumulation in control-KD (green), dysf-KD (red) and dysf-KD embryos co-injected with mOrange1-DysfC (Dysf–mO, yellow). Lack of PS accumulation in dysf-KD myofibers was rescued by Dysf–mO, translation of which is not inhibited by dysf morpholino. (d) Knock-down of anxa6 (anxa6-KD) had no effect on PS accumulation (red) compared with controls (anxa6-control, green). (e) Domain structure of Dysf. (blue AA: predicted amphipathic helix). (f,g), On damage zfWRRFK-TM-C accumulates in the repair patch (f, arrow). No accumulation was observed for zfTM-C (g, arrow). (h) Accumulation kinetics of zfWRRFK-TM-C (red), zfWRRFK-TM (green) and zfTM-C (blue). A 5-AA motif (WRRFK, red box, e) is required for accumulation. (i) zfWRRFK-TM-C (red) but not zfTM-C (green) rescued PS accumulation in dysf-KD myofibers. In all charts, the change of fluorescence at the lesion is indicated as percentage relative to the undamaged state±s.d.). Scale bars, 4 μm (a,g–h), 3 μm (b).

Since a 74 AA C-terminal fragment of Dysf7 was sufficient to restore PS translocation towards the lesion, we next asked which region in Dysf (Fig. 3e) mediates PS accumulation. Thus, we shortened the fragment further to a predicted amphipathic helix29, residing N-terminally of and within the putative TM domain (Fig. 3e, zfWRRFK-TM-C). zfWRRFK-TM-C localized to the Z-lines and sarcolemma and translocated to the repair patch after membrane injury (Fig. 3f,h). Deletion of 14 AAs from the C-terminus (zfWRRFK-TM) did not affect accumulation (Fig. 3e,h, Supplementary Fig. 4g). However, removal of 5 AAs N-terminally led to mislocalization in intact cells and failure to accumulate (Fig. 3g,h, Supplementary Fig. 4g). Strikingly, PS accumulation was not rescued in dysf-morphants co-injected with zfTM-C, while zfWRRFK-TM-C rescued PS enrichment (Fig. 3i, Supplementary Fig. 4h), similar to dysf-mutant embryos (Supplementary Fig. 4i,j). In conclusion, this 5-AA motif which is not related to the reported PS binding C2-domains of Dysf (ref. 5) is essential for the selective accumulation of PS at the repair patch.

Dysf mediates PS relocation

To assess the dynamics of Dysf–mO, we employed fluorescence loss in photobleaching (FLIP). Dysf–mO expressing myofibers were imaged every second for 118 s under a spinning disk laser scanning microscope (Fig. 4a) and damaged by 405-nm laser irradiation. The light exposure also caused substantial bleaching at the site of lesion (Fig. 4a). As a control, we irradiated with 561 nm instead of 405 nm laser light, which only bleaches but does not damage the membrane (Fig. 4b). After local bleaching using 561-nm irradiation, we observed a fluorescence intensity decrease in the region of interest adjacent to the bleached site followed by slow recovery (Fig. 4c). In contrast, after local bleaching and membrane damage by 405-nm irradiation, the fluorescence intensity decreased faster and to a significantly lower level (Fig. 4c, Supplementary Fig. 5a). The faster kinetics and greater extent of fluorescence loss after membrane damage provide strong evidence that Dysf from adjacent, intact sarcolemmal regions is recruited to the site of lesion (Fig. 5a).

Figure 4. Dysf facilitates PS translocation to site of lesion.

Figure 4

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(a–c) Fluorescence loss in photobleaching (FLIP) analysis on selected regions (blue boxes in a,b) of live Dysf–mO expressing myofibers. (a) Photobleaching and membrane damaging using 405 nm laser; (b) photobleaching only using 561 nm laser. (c) 561-nm irradiation results in an intensity decrease by 6% (black curve); 405-nm irradiation results in a more rapid decrease by 14% (red curve). (d–g) Single-molecule trajectory analysis of zfWRRFK-TM-C and controls after sarcolemmal damage. (d) Scheme showing the projection of a single-molecule trajectory onto the line connecting the midpoint with the site of damage. (e) Super-resolution localization image of a mEosFP:zfWRRFK-TM-C labelled myofiber. The arrow indicates the site of lesion. Single-molecules trajectories calculated from the image data in e. Molecules near the lesion (red box) show a high tendency (Rproj.=1.92) to move towards the lesion (red dot). In the undamaged sarcolemma (left), trajectories (black box) did not show directed motion towards the black dot (Rproj.=1.04). (f) mEosFP:zfWRRFK-TM-C moved towards the lesion (red column); the control CAAX-mEosFP did not (green column; P<10−8). (g) The PS sensor LactC2:mEosFP moved towards the lesion in dysf-contr-KD embryos (red column) but not in dysf-KD embryos (green column; Student t-test P<10−8). n⩾8. Scale bar, 5 μm.

Figure 5. The WRRFK-motif is required for accumulation of Dysf and PS.

Figure 5

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(a) After membrane damage, zfWRRFK-TM-C (red) but not zfWAAFK-TM-C (grey) accumulated rapidly at the lesion. The effect was reduced on exchange of the first (zfWARFK-TM-C; purple) or second arginine by alanine (zfWRAFK-TM-C; green). (b) PS (LactC2:GFP) enrichment in the repair patch of Dysf-KD myofibers co-expressing zfWRRFK-TM-C (green), but not of those co-expressing zfWAAFK-TM-C (red). (c–e) HeLa cells expressing zfWRRFK-TM-C were imaged before (c) and 0 s (c, arrow) and 270 s after membrane damage (c, arrows). zfWRRFK-TM-C markedly amassed at the lesion (c, arrow, inset; e, black), whereas zfWAAFK-TM-C showed only baseline fluorescence recovery after photobleaching (d, arrows, inset; e, red). (f–h) HeLa cells transfected with LactC2:GFP and imaged before (f) and 0 s (arrow) and 270 s after lesioning (arrow) showed no PS accumulation but only baseline recovery (f, arrow, inset; h, red); in the presence of zfWRRFK-TM-C, LactC2:GFP accumulated within the repair patch (g, arrows, inset; h, black). The data in (a,b,e,h) are given as mean±s.e.m. (n=>5) and scaled such that 100% corresponds to fluorescence from the same area before damaging. Scale bars, 10 μm (c,d) and 12.86 μm (f,g).

To directly visualize the movement of individual molecules to the wounded area, we performed super-resolution photoactivation localization microscopy, using green-to-red photoconversion of mEosFP (ref. 30). From these data, we analysed the displacements and speeds of 130±5 single-molecule trajectories of mEosFP:zfWRRFK-TM-C along a line connecting the trajectory midpoint and the damage site in regions 2–15 μm away from the wound (Fig. 4d). A parameter, Rproj., was defined revealing random (Rproj.=1) or net directional motion towards (Rproj.>1) or away from (Rproj.<1) the site of lesion (see supplementary experimental procedures). Figure 4e shows a super-resolved image of a mEosFP:zfWRRFK-TM-C labelled myofiber in zebrafish; the arrow indicates the site of damage. The resulting trajectories are shown in Fig. 4e. For zfWRRFK-TM-C, we observed a strong tendency to move towards the damage site (Rproj.=1.92) and to accumulate at the membrane lesion (Fig. 4f). For the undamaged membrane region on the left (Fig. 4b), directional motion is absent (Rproj=1.04), calculated with respect to a randomly chosen point (black dot). Control experiments with lipid-anchored CAAX-mEosFP did not display directional motion in the presence of damage (Fig. 4f, P<10–8), showing that directional motion of zfWRRFK-TM-C was not caused by overall membrane shifts towards the lesion.

We performed additional single-molecule tracking experiments by using the PS sensor LactC2:mEosFP in dysf knock-down and control embryos. LactC2:mEosFP showed a much higher tendency to move towards the lesion in control than in dysf knock-down embryos (Fig. 4g, P<10–8), further supporting our claim that Dysf plays a decisive role in translocating PS within the membrane to the damage site.

Dysf and PS accumulation require arginines

Double arginine (RR) motifs have previously been implicated in Ca2+-independent interaction with PS (ref. 31). Therefore, we tested the relevance of arginines within the WRRFK-motif (Fig. 3e) for accumulation of the Dysf and PS reporters. In contrast to zfWRRFK-TM-C, zfWAAFK-TM-C did not show any enrichment at the lesion (Fig. 5a, Supplementary Fig. 6a). Mutation of either one of the two arginines reduced translocation to the lesion (Fig. 5a, Supplementary Fig. 6a), demonstrating that both contribute to Dysf accumulation (Fig. 5a).

To enquire whether mutating the RR motif affects PS enrichment at the lesion, we knocked-down dysf and expressed LactC2:GFP together with either zfWRRFK-TM-C or zfWAAFK-TM-C. While zfWRRFK-TM-C rescued PS accumulation, dysf-morphants expressing zfWAAFK-TM-C showed only partial rescue (Fig. 5b, Supplementary Fig. 6b).

PS accumulation in human cells

We tested whether zfWRRFK-TM-C accumulates in a heterologous system at injured cell membranes using human HeLa cells expressing very low levels of endogenous DYSF (ref. 32). Membrane damage in zfWRRFK-TM-C expressing HeLa cells (Fig. 5c) resulted in significant enrichment in the lesion patch (Fig. 5c,e), whereas zfWAAFK-TM-C showed no accumulation (Fig. 5d,e, Supplementary Fig. 6c). We examined whether the zebrafish Dysf fragment leads to enrichment of PS at the site of lesion in HeLa cells. Co-expression of LactC2:GFP and zfWRRFK-TM-C led to significant accumulation of PS at membrane lesions (Fig. 5f–h, Supplementary Fig. 6d–f). Undifferentiated C2C12 myoblasts showed LactC2:GFP accumulation only when co-expressing zfWRRFK-TM-C (Supplementary Fig. 6g–j). In contrast, when C2C12 cells were differentiated to myotubes, which express high amounts of endogenous DYSF, LactC2:GFP showed enrichment at the lesion site in the absence of co-transfected zfWRRFK-TM-C (Supplementary Fig. 6k,l). Thus, the C-terminal fragment of zebrafish Dysf is sufficient to mediate PS accumulation at a membrane lesion in human cells, strongly suggesting that the underlying mechanisms are conserved between fish and humans.

Dysferlinopathy caused by mutation of RR motif

A sequence similar to the zebrafish motif WRRFK is present in human Dysf (Fig. 6a, WRRFR). Therefore, we tested whether human WRRFR-TM-C also would accumulate in zebrafish. Indeed, hWRRFR-TM-C showed high enrichment at the lesion (Fig. 6b,c). The double arginine mutant hWAAFR-TM-C showed reduced accumulation compared with hWRRFR-TM-C, yet, enrichment was not completely abolished, suggesting the third arginine may contribute to the activity (Fig. 6c, Supplementary Fig. 7a). On replacement of the third arginine by alanine (hWAAFA-TM-C) or lysine (hWAAFK-TM-C), accumulation was strongly impaired (Fig. 6c, Supplementary Fig. 7a).

Figure 6. Arginine-rich motif is mutated in myopathy patients.

Figure 6

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(a) Zebrafish zfWRRFK-TM-C and human hWRRFR-TM-C (red box arginine-rich motif; black box: TM domain, arrow: R2042C mutation). (b,c), hWRRFR-TM-C localized to the Z-line (z) and sarcolemma (sc) before damage (b). On damage, it accumulated at the lesion (b, arrow; c, red). In contrast, hWAAFK-TM-C (c, blue) and hWAAFA-TM-C (c, yellow) mutants did not accumulate and hWAAFR-TM-C caused significantly reduced accumulation (c, green). (d) LactC2:RFP accumulated in Dysf-KD myofibers when hWRRFR-TM-C (green) but not when mutant hWAAFK-TM-C (red). was co-expressed. (e,f) zfWRCFK-TM-C (e, green) and hWRCFR-TM-C (f, green), modelling the R2042C dysferlinopathic mutation showed significantly reduced accumulation (red). The fluorescence intensity of Dysf reporters at lesion relative to levels before damage is expressed as mean±s.d. (n⩾6). Note that the control data on hWRRFR-TM-C (c,f) are identical. Similarly, zfWRRFK-TM-C data (e) are also shown in Figs 3h and 5a. Scale bar, 4 μm.

Next, we knocked-down dysf and asked whether hWRRFR-TM-C rescued PS accumulation. Indeed, the PS sensor accumulated when hWRRFR-TM-C, but not when mutant hWAAFK-TM-C was co-expressed (Fig. 6d, Supplementary Fig. 7b).

Patients who presented with LGMD2B/MM were reported to have a point mutation in one of the arginines (R2042C, WRCFR)33. Thus, we mutated the corresponding arginine to cysteine (zfWRCFK-TM-C, hWRCFR-TM-C). In agreement with our mutant analysis (Figs 5a and 6c), this modification did not totally abolish, but significantly reduced accumulation of the zebrafish and the human DYSF reporter (Fig. 6e,f, Supplementary Fig. 7c–d). Hence, a likely cause of LGMD2B in these patients is inefficient DYSF and PS accumulation in the repair patch.

Discussion

Lesions in the sarcolemma rapidly lead to formation of repair patches, preventing break-down of the sarcolemmal barrier and cell death. This repair patch has to be removed for restoration of the normal lipid bilayer structure of the sarcolemma. Several mechanisms have been previously suggested for patch removal, including membrane shedding, autophagy and endocytosis11,34,35. We show here that macrophages are key players in membrane repair by phagocytosing the repair patch. First, macrophages associate with and phagocytose material from the membrane patch. Second, knock-down of macrophages abolishes repair patch removal. Third, the repair patch rapidly and selectively accumulates PS a known ‘eat-me' signal for macrophages36. PS is presented at the extracellular side of the repair patch, and thus is accessible to macrophages passing by. Moreover, phagocytosis is delayed when PS is masked.

Repair patch removal by macrophages can occur within minutes in some instances, whereas the clog persisted for hours in other cases. Thus, the repair patch does not seem to produce a long-range attractive signal pointing at the importance of local scanning by macrophages. Whereas small lesions were efficiently repaired, a characteristic sequence of events occurred for larger lesions: formation of a large repair patch, eventually sliding of the repair patch, secondary influx of Ca2+, contraction of the muscle cell, precipitation of AnxAs onto internal membranes, cell death and removal of dead cells by macrophages. Surprisingly, neutrophils were not involved in phagocytosis of repair patches or dead cells. We observed in all cases, however, recruitment of neutrophils to muscle injury by stabbing with a glass needle or tailfin cuts. This suggests that inflammatory signals may play additional roles. The overall much higher inflammatory status may explain the difference to myopathic human skeletal muscle, where neutrophils are heavily engaged37.

Among the lipids tested, only PS and cholesterol accumulated rapidly at lesions, whereas enrichment of general membrane markers was not observed. Our data underscore lipid sorting as mechanism for early repair patch assembly and suggest a key involvement of Dysf in PS-recruitment. Myofibers lacking Dysf failed to accumulate PS. Single-molecule tracking in Dysf morphant and control embryos showed Dysf-dependent, directed movement of PS to the site of lesion. Expression of Dysf fragments in HeLa cells and Dysf-depleted embryos causes PS enrichment. Hence, Dysf sorts PS to the repair patch. It remains to be uncovered whether cholesterol sorting employs the same mechanism.

We noted other lipids, even though not as fast as PS and cholesterol, to accumulate gradually over time. For example, PI(3,4)P2 starts to build up in a delayed manner 4–5 min post lesioning (Supplementary Fig. 7e). This likely reflects membrane flowing in from the edges and providing lipid material to eventually seal the sarcolemmal hole. In this context, systemic exocytosis may be involved expanding the membrane surface of a cell in response to membrane lesions2.

Binding of PS is mediated by the C2A-domains of Dysf (ref. 38). Our data suggest that the arginine-rich motif identified here plays an important additional role in PS transport. Arginine-rich peptides are known to bind PS (ref. 31). Thus, PS may thus directly interact with the Dysf arginine-rich motif.

Mutation of the arginine-rich motif affects localization of Dysf reporters. They appear to be located in the cytoplasm of uninjured myofibers. This suggests that Dysf has to be localized correctly to the sarcolemma to allow PS accumulation. Our single-molecule tracking data reveal that Dysf moves in the plane of the sarcolemma or immediately adjacent and parallel to it. A potential source of Dysf and PS might be caveolae which unfold on membrane stress and thus rapidly increase membrane surface39. We did, however, not observe enrichment of general membrane markers at the lesion excluding overall flux of membrane material towards the lesion as mechanism of PS transport. The mechanism how Dysf and PS are transported towards the lesion remains elusive and needs further investigation. Another key question is how PS is incorporated in the repair patch so that it is exposed to the extracellular space. High local Ca2+ concentrations may inactivate flippases at the lesion as previously proposed in a different context40.

Zebrafish and human DYSF both contain arginine-rich motifs N-terminally of the TM domain. hWRRFR-TM-C accumulated at the membrane patch and rescued PS accumulation in injured zebrafish myofibers, suggesting that these particular functions of Dysf have been conserved during evolution. This claim is supported by complementary approaches expressing the zebrafish Dysf reporter in HeLa cells; zfWRRFK-TM-C accumulated and mediated enrichment of PS at the site of lesion in human cells.

LGMD2B/MM is correlated with a mutation in the arginine-rich motif (R2042C) in human patients33. In accordance, modelling this mutation in both zebrafish and human DYSF fragments revealed significantly lower accumulation at membrane lesions. A disease causing effect of this mutation in humans is thus in complete agreement with our data. Taken together, these human genetic data underscore the physiological significance of the mechanism underlying membrane repair that we uncovered.

Methods

Zebrafish strains

The AB2O2 WT line (European Zebrafish Resource Centre EZRC, Karlsruhe) was used for all experiments. The transgenic lines Tg(mpeg1:GFP) and Tg(LysC:dsRED) were a gift from the Lieschke and the Crosier labs, respectively14,15. Zebrafish husbandry41 and experimental procedures were performed in accordance with German animal protection regulations (Regierungspräsidium Karlsruhe, Germany, AZ35-9185.81/G-137/10).

Expression plasmids and sensors

Cloning was carried out following standard procedures (Supplementary Methods). Muscle expression of sensors was driven by the unc45b promoter (ref. 42). The lipid sensors LactC2:GFP, LactC2:RFP, GFP-2 × PH(PLCδ), AKT-PH:EGFP, Lyn-tailed mCherry-SEpHluorin20,23,24,26, secreted human AnnexinV-YFP reporter28, human DYSF-Venus43, pcDNA3-Clover44 and pEGFP::2FYVE-GFP25 were described previously. Please refer to Supplementary Table 2 for a summary of the sensors used.

Real-time imaging of membrane repair

Plasmids encoding sensors, BODIPY-cholesterol and TopFluor-PS (Avanti Polar Lipids, Alabaster, AL) were injected into the yolk of 1–2 cell embryos45. Levels of expression of sensors did not affect the kinetics of membrane repair processes. Sarcolemmal lesions were generated using 3- to 5-day-old embryos, which were immobilized on a microscopy slide using 0.5% low melting point agarose supplemented with 0.02% MESAB. Embryos were imaged with a water dip-in × 63 objective (NA: 0.90; HCX APO water; Leica) and installed at a Leica TCS SP2 confocal microscope and the corresponding Leica LCS software. The observations were performed at room temperature. The sarcolemma was damaged with a two-photon laser set to 860 nm. Sensor accumulation at the membrane lesion was measured by determining the fluorescence intensity at the lesion in at least five independent experiments. Significance was tested with Welch's test followed by Bonferroni correction using MATLAB.

For imaging (16–22 h), multiple embryos were embedded in LMP agarose (0.5%) in a 6 cm petridish and covered with 10 ml 1 × E3-medium (Supplementary Methods) containing 0.02% MESAB and 0.003% phenylthiourea (PTU). Individual damaged cells were imaged sequentially as Z-stacks of 40–90 μm overnight under an upright TCS SP5 confocal microscope (Leica, HCX PL APO × 20/0.70 lambda blue IMM CORR or HCX APO L × 40/0.80 W U-V-I objectives), using the brightfield and fluorescence channels (488 nm/561 nm).

Knock-down and knock-out

Morpholinos against dysf mRNA7 and for depleting macrophages16,17,18 were used as described (0.8 mM dysf–MO, 0.5 mM pu.1-MO; 0.5 mM gcsfr-MO; 0.6 mM irf8-MO). dysf and irf8 mutants were created by CRISPR/Cas9-mediated stop codon cassette insertion46.

Imaging of HeLa cells

HeLa cells were transfected using Lipofectamine 3000 (Thermo Fisher Scientific, Carlsbad, CA). After 24 h culture at 37 °C, cells were washed with phosphate buffered saline and imaged in DMEM containing 10% foetal bovine serum. Cells were imaged at 37 °C on an Andor Revolution XD spinning disk confocal laser scanning microscope (BFi OPTiLAS, München, Germany) with an OLYMPUS ApoN60 × /1.49 oil immersion objective. The cell membrane was damaged by using the FRAPPA unit to irradiate a region of 6 × 6 pixels with 405 nm laser light at 200 μW on the specimen with a pixel dwell time of 800 μs. Irradiation was repeated 600 times to locally damage the membrane within 18 s. Image sequences were acquired in 10 s intervals, with the first image taken 20 s after membrane damage, followed by analysis with ImageJ.

Super-resolution localization microscopy

Zebrafish myofibers were imaged on a custom-built widefield inverted microscope (Axiovert 200, Zeiss, Göttingen, Germany) with single-molecule sensitivity47, equipped with a C-Apochromat, × 63/1.2 W Corr objective (Zeiss), multiple excitation lasers (405, 473 and 561 nm), an image splitter (Optosplit, Cairn Research Ltd, Faversham, UK) and an EMCCD camera (Ixon Ultra 897, Andor, Belfast, Northern Ireland). Embryos (4–5 dpf) anesthetized with 0.02% MESAB were immobilized on cover glass surfaces in 1% LMP agarose; another cover slip on top held the embryos closely to the bottom surface. Muscle cell membranes were damaged by focusing 405-nm laser light (5 mW on the specimen) for 2–4 s. mEosFP was photoconverted to its red emitting form by 405 nm light and excited at 561 nm. Image stacks were analysed using a-livePALM software48 (Supplementary Methods).

Data availability

All data are provided in the Supplementary Information.

Additional information

How to cite this article: Middel, V. et al. Dysferlin-mediated phosphatidylserine sorting engages macrophages in sarcolemma repair. Nat. Commun. 7:12875 doi: 10.1038/ncomms12875 (2016).

Supplementary Material

Supplementary Information

Supplementary Figures 1-7, Supplementary Tables 1-2, Supplementary Note 1, Supplementary Methods and Supplementary References.

ncomms12875-s1.pdf (934.7KB, pdf)
Supplementary Movie 1

AnxA2a accumulation and Ca2+ spreading in myofibers with lesions =4 μm. AnxA2a-mO (red) was co-expressed with the Ca2+ sensor GCaMP5A (green). After inducing a small wound (=4μm; arrow), the myofiber was monitored by time lapse microscopy for 1200 s in bright field (grey) and fluorescence to detect Ca2+ influx (green) and AnxA2a-mO accumulation (red). Scale bar: 4 μm. Related to Extended Data Fig. 1a-c.

Download video file (4.3MB, avi)
Supplementary Movie 2

Precipitation of AnxA2a preceded by Ca2+ spreading in dying myofibers. AnxA2a-mO (red) was co-expressed with the Ca2+ sensor GCaMP5A (green). After inducing a larger lesion (=4μm; blue arrow), the myofiber was monitored by time lapse microscopy for 1200 s in bright field (grey) and fluorescence to detect Ca2+ release (green) and AnxA2a-mO accumulation (red). The lesion patch slides to the right (purple arrows) and, concomitantly, morphological changes such as formation of large intracellular vesicles (black arrow) and cell shrinkage occur. Finally, the damaged cell contracts and a Ca2+ wave is observed (light blue arrows), followed by AnxA2a-mO precipitation onto internal membranes of the wounded myofiber. Scale bar: 4 μm. Related to Extended Data Fig. 1d-f.

Download video file (13.9MB, avi)
Supplementary Movie 3

Macrophage removing the repair patch. A myofiber and the repair patch were marked with AnxA2a-mO (red); the macrophage expressed Tg(mpeg1:GFP) (green; light blue arrow). Damaged myofibers were recorded by time lapse imaging. AnxA2a-mO (red) accumulates at the lesion (black arrow). A macrophage (green, light blue arrow) close to an undamaged myofiber interacts with the repair patch (black arrow) and phagocytoses it (white arrow). Scale bar: 4 μm. Related to Fig. 1.

Download video file (6.5MB, avi)
Supplementary Movie 4

Macrophages remove dead myofibers. Dead myofibers show precipitation of AnxA2a-mO (red) to internal membranes and are removed by attracted macrophages (green) within the first 600 min after wounding. Scale bar: 4 μm. Related to Extended Data Fig. 2a-c.

Download video file (1.8MB, avi)
Supplementary Movie 5

The PS sensor LactC2:GFP accumulates earlier than AnxA2a. A myofiber expressing LactC2:GFP (green) and AnxA2a-mO (red) is wounded and patch formation (white arrow) is followed by time-lapse imaging. While LactC2:GFP accumulates within the first minute (light blue arrow), AnxA2amO starts to enrich at 60 s post-lesioning (magenta arrow). Scale bar: 4 μm. Related to Fig. 2a.

Download video file (631.7KB, avi)
Supplementary Movie 6

PS is presented to the extracellular space. The PS sensor secA5-YFP (yellow) secreted from neighboring myofibers accumulates at the site of membrane damage of a non-expressing cell (arrow) within the first 282 s. The secA5-YFP secreting myofibers are outside the observation field of the movie. Scale bar: 4 μm. Related to Fig. 3i-l.

Download video file (254.3KB, avi)
Peer Review File
ncomms12875-s8.pdf (1.8MB, pdf)

Acknowledgments

We thank N. Borel and the fish house team, M. Rastegar for microscopy and S. Grinstein, S. Vogel, M. Lin, L.C. Cantley, C. Etard, T. van Ham and R. Peterson for plasmids. We thank T. Dickmeis for critically reading the manuscript. US and GUN were funded by HGF, DFG (STR 439/8-1, Ni 291/12-1), EC IP ZF-HEALTH and BMBF-MIE. In addition, this research work is part of the project `̀Molecular Interaction Engineering: From Nature s Toolbox to Hybrid Technical Systems'', which is funded by the German Federal Ministry of Education and Research (BMBF), funding code 031A095 C.

Footnotes

Author contributions V.M., L.Z., G.U.N and U.S designed the experiments V.M. and L.Z. performed experiments. V.M., U.S. and G.U.N wrote the manuscript. M.R. performed statistical analysis. C.G. provided transgenic zebrafish lines. M.S. helped to clone the calcium sensor. M.T. and T.B. contributed to experimental design and cloning. U.R. provided plasmids, S.R. reviewed the manuscript.

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Supplementary Information

Supplementary Figures 1-7, Supplementary Tables 1-2, Supplementary Note 1, Supplementary Methods and Supplementary References.

ncomms12875-s1.pdf (934.7KB, pdf)
Supplementary Movie 1

AnxA2a accumulation and Ca2+ spreading in myofibers with lesions =4 μm. AnxA2a-mO (red) was co-expressed with the Ca2+ sensor GCaMP5A (green). After inducing a small wound (=4μm; arrow), the myofiber was monitored by time lapse microscopy for 1200 s in bright field (grey) and fluorescence to detect Ca2+ influx (green) and AnxA2a-mO accumulation (red). Scale bar: 4 μm. Related to Extended Data Fig. 1a-c.

Download video file (4.3MB, avi)
Supplementary Movie 2

Precipitation of AnxA2a preceded by Ca2+ spreading in dying myofibers. AnxA2a-mO (red) was co-expressed with the Ca2+ sensor GCaMP5A (green). After inducing a larger lesion (=4μm; blue arrow), the myofiber was monitored by time lapse microscopy for 1200 s in bright field (grey) and fluorescence to detect Ca2+ release (green) and AnxA2a-mO accumulation (red). The lesion patch slides to the right (purple arrows) and, concomitantly, morphological changes such as formation of large intracellular vesicles (black arrow) and cell shrinkage occur. Finally, the damaged cell contracts and a Ca2+ wave is observed (light blue arrows), followed by AnxA2a-mO precipitation onto internal membranes of the wounded myofiber. Scale bar: 4 μm. Related to Extended Data Fig. 1d-f.

Download video file (13.9MB, avi)
Supplementary Movie 3

Macrophage removing the repair patch. A myofiber and the repair patch were marked with AnxA2a-mO (red); the macrophage expressed Tg(mpeg1:GFP) (green; light blue arrow). Damaged myofibers were recorded by time lapse imaging. AnxA2a-mO (red) accumulates at the lesion (black arrow). A macrophage (green, light blue arrow) close to an undamaged myofiber interacts with the repair patch (black arrow) and phagocytoses it (white arrow). Scale bar: 4 μm. Related to Fig. 1.

Download video file (6.5MB, avi)
Supplementary Movie 4

Macrophages remove dead myofibers. Dead myofibers show precipitation of AnxA2a-mO (red) to internal membranes and are removed by attracted macrophages (green) within the first 600 min after wounding. Scale bar: 4 μm. Related to Extended Data Fig. 2a-c.

Download video file (1.8MB, avi)
Supplementary Movie 5

The PS sensor LactC2:GFP accumulates earlier than AnxA2a. A myofiber expressing LactC2:GFP (green) and AnxA2a-mO (red) is wounded and patch formation (white arrow) is followed by time-lapse imaging. While LactC2:GFP accumulates within the first minute (light blue arrow), AnxA2amO starts to enrich at 60 s post-lesioning (magenta arrow). Scale bar: 4 μm. Related to Fig. 2a.

Download video file (631.7KB, avi)
Supplementary Movie 6

PS is presented to the extracellular space. The PS sensor secA5-YFP (yellow) secreted from neighboring myofibers accumulates at the site of membrane damage of a non-expressing cell (arrow) within the first 282 s. The secA5-YFP secreting myofibers are outside the observation field of the movie. Scale bar: 4 μm. Related to Fig. 3i-l.

Download video file (254.3KB, avi)
Peer Review File
ncomms12875-s8.pdf (1.8MB, pdf)

Data Availability Statement

All data are provided in the Supplementary Information.

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