The image for Fig 9 is incorrect. Please see the corrected Fig 9 here.
Fig 9. Differential expression analysis of Pdrg1 silenced clones prepared in H35 cells.
(A) Real-time RT-PCR analysis of Pdrg1 expression using the 18s gene as reference in the stable silenced clones (3–44 and 4–18) and the negative control clone (CN-10) prepared in H35 cells. The results shown are the mean ± SEM of four independent experiments carried out in triplicate. (B) Growth curves for H35 wild type cells (♦), the CN-10 (■) and 4–18 (●) clones; the figure shows the mean ± SEM of eight replicates of a representative independent experiment from the four carried out. (C) Pathway analysis of genes exhibiting expression changes ≥2-fold using Gene Ontology; only pathways with p<0.05 are indicated. (D) Real-time RT-PCR verification of expression changes (mean ± SEM; N = 4) in selected genes using the Rn18s gene as reference.
Reference
- 1.Pérez C, Pérez-Zúñiga FJ, Garrido F, Reytor E, Portillo F, Pajares MA (2016) The Oncogene PDRG1 Is an Interaction Target of Methionine Adenosyltransferases. PLoS ONE 11(8): e0161672 doi:10.1371/journal.pone.0161672 [DOI] [PMC free article] [PubMed] [Google Scholar]

