Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 14;139(10):2751–2765. doi: 10.1093/brain/aww205

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author (2016). Published by Oxford University Press on behalf of the Guarantors of Brain

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0/ ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Reduced CYFIP2 expression does not affect CYFIP1 expression, but leads to increased APP expression at protein but not mRNA level in the hippocampus. ( A ) Representative western blots. ( B ) Quantification showed that CYFIP2 expression is reduced by ∼50% in hippocampal synaptosomes of Cyfip2^+/− mice (black bar; n = 7) in comparison to wild-type littermates (grey bar, n = 8). CYFIP1 levels are not significantly changed in the same lysates. For quantification CYFIP1 and CYFIP2 expression were normalized to levels of NSE, a neuronal marker. ( C ) Representative western blots. ( D ) Quantification showed that APP protein expression is significantly increased ∼2-fold in hippocampal synaptosomes of Cyfip2^+/− mice (black bar) in comparison to wild-type littermates (grey bar; n = 8 per genotype). ( E ) Quantitative PCR analysis showed that App mRNA levels are not elevated in hippocampi of Cyfip2^+/− mice (black bar) in comparison to wild-type littermates (grey bar) when normalized to the housekeeping gene Hprt ( n = 8 per genotype). Means ± SEM are shown. *** P < 0.001.