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. 2016 Apr 18;7(18):24908–24927. doi: 10.18632/oncotarget.8795

Figure 1. Characterization of collagens.

Figure 1

A. Spectrofluorimetric analysis was performed on adult and old collagen to detect AGEs-specific fluorescence expressed as μg/ml. B. CML and C. Pentosidine were quantified by LC-MS/MS and expressed as pmol/mg of collagen. D. Cross-link content was measured by the quantification of hydroxylysylpyridinoline (HLP) and lysylpyrodinoline (LP) by ion exchange chromatography and expressed as μmol (LHP and LP)/mol of collagen. E. SDS-PAGE of collagen samples, 5 μg of either adult or old rat type I collagens were analyzed on 5% polyacrylamide gels under reducing conditions. Collagen chains (α 1 and α 2), and higher-molecular-weight polymers (P) are indicated. Values represent the mean ± S.E.M. of three independent experiments (*p < 0.05, **p < 0.01).