Figure 5. NHEJ plays a predominant role in oxidative stress-induced mutagenesis.

(A) PIG-O mutation analysis in wild type DT40 cells and NHEJ-deficient cells (KU70-, LIGIV-, and DNA PKcs-deficient) exposed to H₂O₂. *p < 0.05 when mutation levels were compared to wild type DT40 cells at respective doses using Student's t-test. (B) Survival of wild type DT40 cells and KU70-, LIGIV-, and DNA PKcs-deficient cells after exposure to H₂O₂, as assessed during the phenotype expression period in the mutation assay. (C) PIG-O mutation analysis in wild type DT40 cells and KU70- deficient cells exposed to methylmethanesulfonate. Data represent mean (n = 3) ± SD.