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. 2016 Mar 23;7(18):25885–25901. doi: 10.18632/oncotarget.8292

Figure 1. Trabectedin and lurbinectedin activate both ATM and ATR pathways.

Figure 1

A. HeLa cells were mock-treated or exposed to 20 nM trabectedin (left panel) or lurbinectedin (right panel) for 1 hour followed by 6 hours post-incubation in drug-free media. Cells were then processed for immunolabeling with an antibody directed against Ser1981-phosphorylated ATM. Fluorescence intensities in individual cells were quantified by Metamorph analysis and are indicated in arbitrary units (a.u.). B. Same as above except than the cells were pre-permeabilized with ice-cold CSK-lysis buffer to remove the soluble fraction of RPA32 before processing for immunolabeling with an antibody directed against RPA32. C. Same as above except than cells were processed for simultaneous staining of Ser1981-phosphorylated ATM and RPA32. ρ denotes the correlation coefficient between the intensities of Ser1981-phosphorylated ATM and RPA32.