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. 2016 Mar 23;7(18):25885–25901. doi: 10.18632/oncotarget.8292

Figure 2. Both trabectedin and lurbinectedin induce the DNA damage response.

Figure 2

A. HeLa cells were mock-treated (UT) or incubated for 1 hour with trabectedin (20 nM) followed by 0, 2, 4, 6 hours post-incubation in drug-free media as indicated. Total protein extracts were prepared and analyzed by immunolabeling with antibodies directed against Ser317-phosphorylated Chk1, Thr68-phosphorylated Chk2, Thr21-phosphorylated RPA32 and Ser139-phosphorylated H2AX. Total Chk1, Chk2, RPA32 and H2AX were used as loading controls. B. HeLa cells were either untreated (UT) or incubated for 1 hour with 20 nM trabectedin (T) or lurbinectedin (L) followed by 6 hours post-incubation in drug-free media. Total protein extracts were prepared and analyzed by immunolabeling with antibodies directed against Ser317-phosphorylated Chk1, Thr68-phosphorylated Chk2 and RPA32. Total Chk1 and Chk2 were used as loading controls. On each panel, arrows indicate the main phosphorylated forms of RPA32.