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. 2016 Mar 23;7(18):25915–25929. doi: 10.18632/oncotarget.8297

Figure 6. Reduced Arnt expression impairs AhR-mediated gene expression and DNA damage.

Figure 6

A. Arnt, B. Cyp1a1, C. Cyp1b1, and D. Aldh3a1 mRNA expression in bone marrow-derived macrophages (BMDMs) transiently transfected with siRNA against Arnt (siArnt) or control siRNA (siCtrl), followed by stimulation with MCA for 8 h, assessed by qPCR. mRNA levels were normalized to Tbp. The ratio of Arnt, Cyp1a1, Cyp1b1, or Aldh3a1 and Tbp in siCtrl BMDMs after MCA-stimulation was set to 1. Values are the mean ± SEM of n = 4 (each n containing 3 mice/genotype), * P < 0.05 compared to siCtrl MCA. E. Illustration of the coculture of NIH3T3 cells with wt BMDMs that were transfected with siRNA against Arnt (siArnt) and control siRNA (siCtrl) and incubated with MCA for 24 h. Representative Western analysis of phosphorylated γH2AX in NIH3T3 cells from 4 experiments. Nucleolin served as a loading control.