Figure 6. Salidroside inhibits inflammation induced by SUV irradiation in vivo.
A. Salidroside inhibited inflammation induced by SUV and down-regulated COX-2 level, phosphorylationof P38 and JNKs in mouse skin. Adult Babl/c mice were irradiated with one dose of solar UV light (100 KJ/m2) after pre-treatment of Salidroside or acetone in the dorsal skin for 3 h, and dorsal trunk skin samples for H&E staining and IHC were harvested at 24 h after irradiation. The levels of COX-2, phosphorylation of P38 or JNKs were shown mainly in epidermas of mice skin. B. Quantification of COX-2 expression, phosphorylation levels of p38, or JNKs were analyzed by the Medicine Image Analysis System (MIAS) and data are shown as the average optical density (AOD). Data were presented as means ± SEM of values from ten samples. The magnification of representative photos for H&E and the immunohistochemical staining was 20 ×. C. and D. The secretion of PGE2, IL-6 and TNF-α were significantly reduced by salidroside treatment in mouse skin tissues. Data were shown as mean ± SEM and asterisks indicated a significant inhibition by salidroside compared with the group treated with SUV alone (*P<0.05; ***P<0.001).