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. 2016 Mar 30;7(18):26388–26399. doi: 10.18632/oncotarget.8506

Figure 3. Caspase-2 is a functional target of miR-149 in U87-MG and A172 cells.

Figure 3

(A) Alignment of potential miR-149-binding sites in the 3′UTR of the caspase-2 mRNA. Schematic shows the 3′UTR sequences of mature miR-149 and the seed mutant region. The seed 3′UTR sequence of mature miR-149 binding is underlined. (B) Luciferase activity assays showing decreased reporter activity after co-transfection of either wild type caspase-2–3′UTR or it's mutated 3′UTR with miR-149 in U87-MG cells. (C) HEK293 cells co-transfected with caspase-2–3′UTR and anti-miR-149 showing increasing reporter activity that is concentration-dependent. (D) Western blot and RT-PCR analysis showing miR-149 suppression of caspase-2 expression in U87-MG and A172 cell lines. (E) Caspase-2 expression was enhanced after miR-149 inhibition in U87-MG and A172 cell lines. *p < 0.05; **p < 0.01.