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. 2016 Mar 30;7(18):26388–26399. doi: 10.18632/oncotarget.8506

Figure 4. Caspase-2 deficiency caused by miR-149 expression contributes to temozolomide-induced apoptosis resistance via p21 and p53 inhibition.

Figure 4

(A) RT-PCR shows relative caspase-2 expression after caspase-2 knockdown by its siRNA. (B) Western blot shows relative caspase-2 expression after caspase-2 knockdown by its siRNA. (C). Apoptosis induced by temozolomide was augmented by caspase-2 expression. Proliferation assay showing inhibition of proliferation after caspase-2 over expression in U87-MG cells. (D) siRNA of caspase-2 showed down regulating p53 and p21 expression. (E) Relative p21 expression were decreased after miR-149 expression in U87-MG cells. (F) miR-149 expression down-regulated p53, Wee1 expression, while phosphorylation of CDC-2 was activated in U87-MG cells.*p < 0.05; **p < 0.01.