Skip to main content
. 2016 Apr 6;7(18):26898–26915. doi: 10.18632/oncotarget.8616

Figure 4. DNA binding domain of p53 interacts with the C-terminal domain of PG.

Figure 4

Equal amounts of total cell extracts from double transfectants coexpressing HA-p53-WT and various FLAG-tagged PG proteins (A) or FLAG-PG-WT and various HA-tagged p53 proteins (B) were processed for reciprocal and sequential immunoprecipitation and immunoblotting using HA and FLAG antibodies as described in Materials and Methods. The immune complexes in A were separated on 7.5%, and in B on 5-20% SDS gradient gels. PG, plakoglobin; WT, wild type; N, N-terminus; C, C-terminus; Arm, Armadillo; DBD, DNA binding domain.