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Primary DCs from DC-cKO mice lack GFP fluorescence. Single cells were recovered from (A) Int, (B) LP, (C) mLNs and (D) Spl from both parental mouse strains, CD11c-CRE-GFP (11c) and Mgat2fl/fl (Mg), and DC-cKO (cKO) mice, and stained for CD11c. Flow cytometric analysis of these cells by GFP fluorescence revealed undetectable to low signal above nonfluorescent cell controls (Mgat2fl/fl) (representative plots of n = 3 mice are shown).
