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Varied tissue DCs show modest Mgat2 ablation penetrance. Single cells were recovered from (A) Int, (B) LP, (C) mLNs and (D) Spl from WT parental mice (CD11c-CRE-GFP) and DC-cKO (cKO) mice, and stained for CD11c and complex N-glycans with PHA-L. Representative histograms of CD11c+ gated cells and replicates (E) show the percentage of PHA-L− cells ranging from 23.9% to 34.2% DC-cKO mice compared to 1.6% to 4.3% in WT mice (n = 3; P < 0.001 compared to WT cells).
