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. 1992 Nov 15;89(22):11002–11005. doi: 10.1073/pnas.89.22.11002

Signal-mediated nuclear transport in simian virus 40-transformed cells is regulated by large tumor antigen.

C M Feldherr 1, R E Lanford 1, D Akin 1
PMCID: PMC50471  PMID: 1279701

Abstract

Transformation of cultured cells with simian virus 40 (SV40), or transfection with the early region of the SV40 genome, causes a significant increase in both the rate of signal-mediated nuclear transport and the functional size of the transport channels (located in the pore complexes). By microinjecting purified large tumor (T) antigen into the cytoplasm of murine BALB/c 3T3 cells, we have demonstrated that this protein alone can account for the increase in transport capacity. The T antigen-dependent changes can be partially inhibited by cycloheximide and require a functional nuclear localization sequence. Although necessary, the nuclear localization sequence by itself cannot produce the observed variations in nuclear permeability and presumably function in a "helper" capacity, in association with another, as yet unidentified domain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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