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. 1994 Apr;78(4):302–306. doi: 10.1136/bjo.78.4.302

Effects of irrigation solutions on corneal endothelial function.

M I Yagoubi 1, W J Armitage 1, J Diamond 1, D L Easty 1
PMCID: PMC504768  PMID: 8199119

Abstract

Rabbit corneas were perfused in vitro with an irrigation solution for 90 minutes. This was followed by 6 hours of perfusion with tissue culture medium TC199 during which endothelial function was assessed by monitoring rates of swelling during a period of perfusion in the absence of bicarbonate ions, and subsequent rates of thinning when bicarbonate ions were restored to the perfusate. Corneal thickness (measured with an ultrasonic pachymeter) immediately following excision was 401 microns (SD 19, n = 23). During the 90 minute perfusion at 35 degrees C, corneas exposed to balanced salt solution (BSS), Hartmann's solution or 0.9% NaCl (all initially at room temperature) swelled, respectively, at 14 (SD 2.3, n = 4), 11 (SD 2.6, n = 4), and 70 (SD 4.3, n = 4) microns/h. Cold Hartmann's solution (initially at 4 degrees C) caused corneas to swell at 9 (SD 2.3, n = 4) microns/h. On the other hand, corneas perfused with BSS Plus thinned at 9 (SD 3.4, n = 4) microns/h and TC199 with Earle's salts had little effect on thickness. Rates of swelling and thinning during the following assessment perfusion showed no apparent effects of prior exposure to any of the irrigation solutions on the barrier properties or pump function of the endothelium. Despite this, the increased thickness of corneas exposed initially to BSS, cold Hartmann's solution, or 0.9% NaCl was not fully reversed, even by the end of the 6 hour assessment perfusion. In contrast, the swelling observed in corneas exposed to Hartmann's solution at room temperature was reversed and these corneas had returned to their normal thickness by the end of the assessment period. All corneas, even those exposed to 0.9% NaCl, had an intact endothelial mosaic with no evidence of damage or cell loss, although morphological differences in cell shape and the appearance of cell borders were evident compared with freshly isolated cornea.

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Selected References

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  1. Benson W. E., Diamond J. G., Tasman W. Intraocular irrigating solutions for pars plana vitrectomy. A prospective, randomized, double-blind study. Arch Ophthalmol. 1981 Jun;99(6):1013–1015. doi: 10.1001/archopht.1981.03930011013005. [DOI] [PubMed] [Google Scholar]
  2. Burke M. J., Parks M. M., Calhoun J. H., Diamond J. G., deFaller J. M. Safety evaluation of BSS plus in pediatric intraocular surgery. J Pediatr Ophthalmol Strabismus. 1981 May-Jun;18(3):45–49. doi: 10.3928/0191-3913-19810501-11. [DOI] [PubMed] [Google Scholar]
  3. Dikstein S., Maurice D. M. The metabolic basis to the fluid pump in the cornea. J Physiol. 1972 Feb;221(1):29–41. doi: 10.1113/jphysiol.1972.sp009736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edelhauser H. F., Gonnering R., Van Horn D. L. Intraocular irrigating solutions. A comparative study of BSS Plus and lactated Ringer's solution. Arch Ophthalmol. 1978 Mar;96(3):516–520. doi: 10.1001/archopht.1978.03910050284021. [DOI] [PubMed] [Google Scholar]
  5. Edelhauser H. F., Van Horn D. L., Hyndiuk R. A., Schultz R. O. Intraocular irrigating solutions. Their effect on the corneal endothelium. Arch Ophthalmol. 1975 Aug;93(8):648–657. doi: 10.1001/archopht.1975.01010020614011. [DOI] [PubMed] [Google Scholar]
  6. Elliott G. F., Goodfellow J. M., Woolgar A. E. Swelling studies of bovine corneal stroma without bounding membranes. J Physiol. 1980 Jan;298:453–470. doi: 10.1113/jphysiol.1980.sp013094. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Glasser D. B., Matsuda M., Ellis J. G., Edelhauser H. F. Effects of intraocular irrigating solutions on the corneal endothelium after in vivo anterior chamber irrigation. Am J Ophthalmol. 1985 Mar 15;99(3):321–328. doi: 10.1016/0002-9394(85)90363-0. [DOI] [PubMed] [Google Scholar]
  8. Gonnering R., Edelhauser H. F., Van Horn D. L., Durant W. The pH tolerance of rabbit and human corneal endothelium. Invest Ophthalmol Vis Sci. 1979 Apr;18(4):373–390. [PubMed] [Google Scholar]
  9. Hodson S., Miller F. The bicarbonate ion pump in the endothelium which regulates the hydration of rabbit cornea. J Physiol. 1976 Dec;263(3):563–577. doi: 10.1113/jphysiol.1976.sp011645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaye G. I., Mishima S., Cole J. D., Kaye N. W. Studies on the cornea. VII. Effects of perfusion with a Ca++-free medium on the corneal endothelium. Invest Ophthalmol. 1968 Feb;7(1):53–66. [PubMed] [Google Scholar]
  11. Kramer K. K., Thomassen T., Evaul J. Intraocular irrigating solutions: a clinical study of BSS plus and dextrose bicarbonate lactated Ringer's solution. Ann Ophthalmol. 1991 Mar;23(3):101–105. [PubMed] [Google Scholar]
  12. Matsuda M., Tano Y., Edelhauser H. F. Comparison of intraocular irrigating solutions used for pars plana vitrectomy and prevention of endothelial cell loss. Jpn J Ophthalmol. 1984;28(3):230–238. [PubMed] [Google Scholar]
  13. McCarey B. E., Edelhauser H. F., Van Horn D. L. Functional and structural changes in the corneal edothelium during in vitro perfusion. Invest Ophthalmol. 1973 Jun;12(6):410–417. [PubMed] [Google Scholar]
  14. McDermott M., Snyder R., Slack J., Holley G., Edelhauser H. Effects of intraocular irrigants on the preserved human corneal endothelium. Cornea. 1991 Sep;10(5):402–407. doi: 10.1097/00003226-199109000-00008. [DOI] [PubMed] [Google Scholar]
  15. Moorhead L. C., Redburn D. A., Merritt J., Garcia C. A. The effects of intravitreal irrigation during vitrectomy on the electroretinogram. Am J Ophthalmol. 1979 Aug;88(2):239–245. doi: 10.1016/0002-9394(79)90472-0. [DOI] [PubMed] [Google Scholar]
  16. Rosenfeld S. I., Waltman S. R., Olk R. J., Gordon M. Comparison of intraocular irrigating solutions in pars plana vitrectomy. Ophthalmology. 1986 Jan;93(1):109–115. doi: 10.1016/s0161-6420(86)33790-4. [DOI] [PubMed] [Google Scholar]
  17. Taylor M. J., Hunt C. J. Dual staining of corneal endothelium with trypan blue and alizarin red S: importance of pH for the dye-lake reaction. Br J Ophthalmol. 1981 Dec;65(12):815–819. doi: 10.1136/bjo.65.12.815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Whikehart D. R., Edelhauser H. F. Glutathione in rabbit corneal endothelia: the effects of selected perfusion fluids. Invest Ophthalmol Vis Sci. 1978 May;17(5):455–464. [PubMed] [Google Scholar]

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