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. 1994 Aug;78(8):632–637. doi: 10.1136/bjo.78.8.632

Localisation of alpha(2,3) and alpha(2,6) linked terminal sialic acid groups in human trabecular meshwork.

S A Chapman 1, R E Bonshek 1, R W Stoddart 1, K R Mackenzie 1, D McLeod 1
PMCID: PMC504889  PMID: 7918292

Abstract

Sialic acid specific lectins were used to localise isomers of sialyl glycosides in human trabecular meshwork (TM) at the ultrastructural level. A lectin immunogold method demonstrated that sialic groups were concentrated on the endothelial surface of Schlemm's canal (SC) and in the adjacent juxta-canalicular tissue (JCT). One sialyl glycoside, alpha(2,6) linked N-acetyl neuraminic acid, was present mainly on the luminal aspect of the SC endothelium and in the cytoplasm of the JCT cells. Another, alpha(2,3) linked N-acetyl neuraminic acid, was localised predominantly to the extracellular fibrillar material of the JCT. The existence of a topographical segregation of these two sialyl glycosides within the TM supports the view that highly charged anionic molecules may be of significance in regulating aqueous outflow.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armaly M. F., Wang Y. Demonstration of acid mucopolysaccharides in the trabecular meshwork of the Rhesus monkey. Invest Ophthalmol. 1975 Jul;14(7):507–516. [PubMed] [Google Scholar]
  2. Bill A. Some aspects of aqueous humour drainage. Eye (Lond) 1993;7(Pt 1):14–19. doi: 10.1038/eye.1993.4. [DOI] [PubMed] [Google Scholar]
  3. Blau E. B., Haas J. E. Glomerular sialic acid and proteinuria in human renal disease. Lab Invest. 1973 Apr;28(4):477–481. [PubMed] [Google Scholar]
  4. Carreras F. J., López Caballero J. J., Porcel D. A gel of glycosaminoglycans lining the anterior and posterior chambers in man: histochemical evidence at light and electron microscopy levels. Eye (Lond) 1992;6(Pt 6):574–582. doi: 10.1038/eye.1992.125. [DOI] [PubMed] [Google Scholar]
  5. De Bruyn P. P., Michelson S. Changes in the random distribution of sialic acid at the surface of the myeloid sinusoidal endothelium resulting from the presence of diaphragmed fenestrae. J Cell Biol. 1979 Sep;82(3):708–714. doi: 10.1083/jcb.82.3.708. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Epstein D. L., Rohen J. W. Morphology of the trabecular meshwork and inner-wall endothelium after cationized ferritin perfusion in the monkey eye. Invest Ophthalmol Vis Sci. 1991 Jan;32(1):160–171. [PubMed] [Google Scholar]
  7. Floyd B. B., Cleveland P. H., Worthen D. M. Fibronectin in human trabecular drainage channels. Invest Ophthalmol Vis Sci. 1985 Jun;26(6):797–804. [PubMed] [Google Scholar]
  8. Grierson I., Lee W. R. Acid mucopolysaccharides in the outflow apparatus. Exp Eye Res. 1975 Nov;21(5):417–431. doi: 10.1016/0014-4835(75)90124-4. [DOI] [PubMed] [Google Scholar]
  9. Grierson I., Lee W. R. Light microscopic quantitation of the endothelial vacuoles in Schlemm's canal. Am J Ophthalmol. 1977 Aug;84(2):234–246. doi: 10.1016/0002-9394(77)90857-1. [DOI] [PubMed] [Google Scholar]
  10. Inomata H., Bill A., Smelser G. K. Aqueous humor pathways through the trabecular meshwork and into Schlemm's canal in the cynomolgus monkey (Macaca irus). An electron microscopic study. Am J Ophthalmol. 1972 May;73(5):760–789. doi: 10.1016/0002-9394(72)90394-7. [DOI] [PubMed] [Google Scholar]
  11. Kanwar Y. S., Farquhar M. G. Detachment of endothelium and epithelium from the glomerular basement membrane produced by kidney perfusion with neuraminidase. Lab Invest. 1980 Mar;42(3):375–384. [PubMed] [Google Scholar]
  12. Kerjaschki D., Vernillo A. T., Farquhar M. G. Reduced sialylation of podocalyxin--the major sialoprotein of the rat kidney glomerulus--in aminonucleoside nephrosis. Am J Pathol. 1985 Mar;118(3):343–349. [PMC free article] [PubMed] [Google Scholar]
  13. Knibbs R. N., Goldstein I. J., Ratcliffe R. M., Shibuya N. Characterization of the carbohydrate binding specificity of the leukoagglutinating lectin from Maackia amurensis. Comparison with other sialic acid-specific lectins. J Biol Chem. 1991 Jan 5;266(1):83–88. [PubMed] [Google Scholar]
  14. Knibbs R. N., Perini F., Goldstein I. J. Structure of the major concanavalin A reactive oligosaccharides of the extracellular matrix component laminin. Biochemistry. 1989 Jul 25;28(15):6379–6392. doi: 10.1021/bi00441a034. [DOI] [PubMed] [Google Scholar]
  15. Kolínská J., Baudysová M., Zákostelecká M., Kraml J., Kadlecová L. Regulation of sialylation of intestinal brush-border enzymes and of sialyltransferase activity in organ cultures by dexamethasone. Biochem Int. 1990 Nov;22(3):495–508. [PubMed] [Google Scholar]
  16. Marshall G. E., Konstas A. G., Lee W. R. Immunogold localization of type IV collagen and laminin in the aging human outflow system. Exp Eye Res. 1990 Dec;51(6):691–699. doi: 10.1016/0014-4835(90)90054-x. [DOI] [PubMed] [Google Scholar]
  17. Marshall G. E., Konstas A. G., Lee W. R. Immunogold ultrastructural localization of collagens in the aged human outflow system. Ophthalmology. 1991 May;98(5):692–700. doi: 10.1016/s0161-6420(91)32232-2. [DOI] [PubMed] [Google Scholar]
  18. Miller R. L., Collawn J. F., Jr, Fish W. W. Purification and macromolecular properties of a sialic acid-specific lectin from the slug Limax flavus. J Biol Chem. 1982 Jul 10;257(13):7574–7580. [PubMed] [Google Scholar]
  19. Mäepea O., Bill A. Pressures in the juxtacanalicular tissue and Schlemm's canal in monkeys. Exp Eye Res. 1992 Jun;54(6):879–883. doi: 10.1016/0014-4835(92)90151-h. [DOI] [PubMed] [Google Scholar]
  20. Quinton P. M., Philpott C. W. A role for anionic sites in epithelial architecture. Effects of cationic polymers on cell membrane structure. J Cell Biol. 1973 Mar;56(3):787–796. doi: 10.1083/jcb.56.3.787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rohen J. W., Futa R., Lütjen-Drecoll E. The fine structure of the cribriform meshwork in normal and glaucomatous eyes as seen in tangential sections. Invest Ophthalmol Vis Sci. 1981 Oct;21(4):574–585. [PubMed] [Google Scholar]
  22. Schauer R. Chemistry, metabolism, and biological functions of sialic acids. Adv Carbohydr Chem Biochem. 1982;40:131–234. doi: 10.1016/s0065-2318(08)60109-2. [DOI] [PubMed] [Google Scholar]
  23. Shibuya N., Goldstein I. J., Broekaert W. F., Nsimba-Lubaki M., Peeters B., Peumans W. J. The elderberry (Sambucus nigra L.) bark lectin recognizes the Neu5Ac(alpha 2-6)Gal/GalNAc sequence. J Biol Chem. 1987 Feb 5;262(4):1596–1601. [PubMed] [Google Scholar]
  24. Taatjes D. J., Roth J., Peumans W., Goldstein I. J. Elderberry bark lectin--gold techniques for the detection of Neu5Ac (alpha 2,6) Gal/GalNAc sequences: applications and limitations. Histochem J. 1988 Sep;20(9):478–490. doi: 10.1007/BF01002646. [DOI] [PubMed] [Google Scholar]
  25. Tawara A., Varner H. H., Hollyfield J. G. Distribution and characterization of sulfated proteoglycans in the human trabecular tissue. Invest Ophthalmol Vis Sci. 1989 Oct;30(10):2215–2231. [PubMed] [Google Scholar]
  26. Tripathi B. J., Millard C. B., Tripathi R. C. Qualitative and quantitative analyses of sialic acid in the human trabecular meshwork. Exp Eye Res. 1990 Nov;51(5):601–606. doi: 10.1016/0014-4835(90)90091-8. [DOI] [PubMed] [Google Scholar]
  27. Tripathi R. C., Tripathi B. J., Spaeth G. L. Localization of sialic acid moieties in the endothelial lining of Schlemm's canal in normal and glaucomatous eyes. Exp Eye Res. 1987 Feb;44(2):293–306. doi: 10.1016/s0014-4835(87)80013-1. [DOI] [PubMed] [Google Scholar]
  28. Tripathi R. C. Ultrastructure of Schlemm's canal in relation to aqueous outflow. Exp Eye Res. 1968 Jul;7(3):335–341. doi: 10.1016/s0014-4835(68)80047-8. [DOI] [PubMed] [Google Scholar]
  29. Troy F. A., 2nd Polysialylation: from bacteria to brains. Glycobiology. 1992 Feb;2(1):5–23. doi: 10.1093/glycob/2.1.5. [DOI] [PubMed] [Google Scholar]
  30. Wang X. C., Smith T. J., Lau J. T. Transcriptional regulation of the liver beta-galactoside alpha 2,6-sialyltransferase by glucocorticoids. J Biol Chem. 1990 Oct 15;265(29):17849–17853. [PubMed] [Google Scholar]
  31. Yun A. J., Murphy C. G., Polansky J. R., Newsome D. A., Alvarado J. A. Proteins secreted by human trabecular cells. Glucocorticoid and other effects. Invest Ophthalmol Vis Sci. 1989 Sep;30(9):2012–2022. [PubMed] [Google Scholar]

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