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. 1994 Nov;78(11):813–817. doi: 10.1136/bjo.78.11.813

Molecular epidemiology of trachoma in a Gambian village.

R L Bailey 1, L Hayes 1, M Pickett 1, H C Whittle 1, M E Ward 1, D C Mabey 1
PMCID: PMC504962  PMID: 7848974

Abstract

The application of a diagnostic and genotyping technique based on the polymerase chain reaction (PCR) to the study of trachoma epidemiology in the Gambian village of Jali is reported. PCR based on the major outer membrane protein (MOMP) gene of Chlamydia trachomatis appears to be more sensitive than either isolation or antigen detection by enzyme immunoassay; it had a specificity of 95% and sensitivity of 51% against clinical signs. PCR genotyping identified genotypes A and B of Chlamydia trachomatis circulating in Jali. Sequencing revealed a Pst1 restriction endonuclease site in the amplified MOMP gene of some B strains but not others; Pst1 digestion of the PCR product proved an easy method of distinguishing these strains. The distribution of serotypes and B strain variants shows a significant degree of household clustering (p < 0.001). PCR based genotyping combined with strain typing provides a new and powerful epidemiological tool for the study of transmission events in trachoma.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailey R., Osmond C., Mabey D. C., Whittle H. C., Ward M. E. Analysis of the household distribution of trachoma in a Gambian village using a Monte Carlo simulation procedure. Int J Epidemiol. 1989 Dec;18(4):944–951. doi: 10.1093/ije/18.4.944. [DOI] [PubMed] [Google Scholar]
  2. Barenfanger J. Studies on the role of the famiy unit in the transmission of trachoma. Am J Trop Med Hyg. 1975 May;24(3):509–515. doi: 10.4269/ajtmh.1975.24.509. [DOI] [PubMed] [Google Scholar]
  3. Bobo L., Munoz B., Viscidi R., Quinn T., Mkocha H., West S. Diagnosis of Chlamydia trachomatis eye infection in Tanzania by polymerase chain reaction/enzyme immunoassay. Lancet. 1991 Oct 5;338(8771):847–850. doi: 10.1016/0140-6736(91)91502-l. [DOI] [PubMed] [Google Scholar]
  4. Claas H. C., Melchers W. J., de Bruijn I. H., de Graaf M., van Dijk W. C., Lindeman J., Quint W. G. Detection of Chlamydia trachomatis in clinical specimens by the polymerase chain reaction. Eur J Clin Microbiol Infect Dis. 1990 Dec;9(12):864–868. doi: 10.1007/BF01967500. [DOI] [PubMed] [Google Scholar]
  5. Dean D., Schachter J., Dawson C. R., Stephens R. S. Comparison of the major outer membrane protein variant sequence regions of B/Ba isolates: a molecular epidemiologic approach to Chlamydia trachomatis infections. J Infect Dis. 1992 Aug;166(2):383–392. doi: 10.1093/infdis/166.2.383. [DOI] [PubMed] [Google Scholar]
  6. Grayston J. T., Wang S. P., Yeh L. J., Kuo C. C. Importance of reinfection in the pathogenesis of trachoma. Rev Infect Dis. 1985 Nov-Dec;7(6):717–725. doi: 10.1093/clinids/7.6.717. [DOI] [PubMed] [Google Scholar]
  7. Hayes L. J., Bailey R. L., Mabey D. C., Clarke I. N., Pickett M. A., Watt P. J., Ward M. E. Genotyping of Chlamydia trachomatis from a trachoma-endemic village in the Gambia by a nested polymerase chain reaction: identification of strain variants. J Infect Dis. 1992 Nov;166(5):1173–1177. doi: 10.1093/infdis/166.5.1173. [DOI] [PubMed] [Google Scholar]
  8. Holland S. M., Hudson A. P., Bobo L., Whittum-Hudson J. A., Viscidi R. P., Quinn T. C., Taylor H. R. Demonstration of chlamydial RNA and DNA during a culture-negative state. Infect Immun. 1992 May;60(5):2040–2047. doi: 10.1128/iai.60.5.2040-2047.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mabey D. C., Robertson J. N., Ward M. E. Detection of Chlamydia trachomatis by enzyme immunoassay in patients with trachoma. Lancet. 1987 Dec 26;2(8574):1491–1492. doi: 10.1016/s0140-6736(87)92623-7. [DOI] [PubMed] [Google Scholar]
  10. Ostergaard L., Birkelund S., Christiansen G. Use of polymerase chain reaction for detection of Chlamydia trachomatis. J Clin Microbiol. 1990 Jun;28(6):1254–1260. doi: 10.1128/jcm.28.6.1254-1260.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Phillips S. E., Bradley J. S. Bacteremia detected by lysis direct plating in a neonatal intensive care unit. J Clin Microbiol. 1990 Jan;28(1):1–4. doi: 10.1128/jcm.28.1.1-4.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schachter J., Moncada J., Dawson C. R., Sheppard J., Courtright P., Said M. E., Zaki S., Hafez S. F., Lorincz A. Nonculture methods for diagnosing chlamydial infection in patients with trachoma: a clue to the pathogenesis of the disease? J Infect Dis. 1988 Dec;158(6):1347–1352. doi: 10.1093/infdis/158.6.1347. [DOI] [PubMed] [Google Scholar]
  13. Taylor H. R., Rapoza P. A., West S., Johnson S., Munoz B., Katala S., Mmbaga B. B. The epidemiology of infection in trachoma. Invest Ophthalmol Vis Sci. 1989 Aug;30(8):1823–1833. [PubMed] [Google Scholar]
  14. Taylor H. R., Siler J. A., Mkocha H. A., Munoz B., West S. The natural history of endemic trachoma: a longitudinal study. Am J Trop Med Hyg. 1992 May;46(5):552–559. doi: 10.4269/ajtmh.1992.46.552. [DOI] [PubMed] [Google Scholar]
  15. Thylefors B., Dawson C. R., Jones B. R., West S. K., Taylor H. R. A simple system for the assessment of trachoma and its complications. Bull World Health Organ. 1987;65(4):477–483. [PMC free article] [PubMed] [Google Scholar]
  16. Treharne J. D. The microbial epidemiology of trachoma. Int Ophthalmol. 1988;12(1):25–29. doi: 10.1007/BF00133777. [DOI] [PubMed] [Google Scholar]
  17. Ward M., Bailey R., Lesley A., Kajbaf M., Robertson J., Mabey D. Persisting inapparent chlamydial infection in a trachoma endemic community in The Gambia. Scand J Infect Dis Suppl. 1990;69:137–148. [PubMed] [Google Scholar]

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