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. 1994 Dec;78(12):927–932. doi: 10.1136/bjo.78.12.927

S-antigen specific T cell clones from a patient with Behçet's disease.

J H Yamamoto 1, Y Fujino 1, C Lin 1, M Nieda 1, T Juji 1, K Masuda 1
PMCID: PMC504995  PMID: 7529558

Abstract

The isolation and characterisation of T cell clones or lines specific to retinal antigens are valuable tools to clarify the underlying mechanisms of autoimmunity to retinal antigens as a contributing factor in ocular inflammation. Patients with Behçet's disease have been reported to be sensitised to S-antigen (S-Ag). In the present study, four T cell clones established from the peripheral blood of a patient with Behçet's disease were analysed. A CD4+ T cell clone (clone 2) and a CD8+ T cell clone (clone 10) proliferated specifically to bovine S-Ag. Although these S-Ag specific T cell clones proliferated vigorously to the intact antigen, their responses to S-Ag derived synthetic peptides M and G were weak, suggesting that the sites of human T cell recognition of S-Ag may be different from those established in the experimental model. The proliferative responses of both clones (2 and 10) were inhibited by anti-HLA-DR monoclonal antibody but not by anti-HLA-class 1 monoclonal antibody. The other two clones studied, clones 6 and 30, were CD3+, CD4-, CD8-, and they did not proliferate specifically to S-Ag. Clone 6 expressed gamma delta T cell receptors (TCR) and showed non-specific cytotoxic activity toward K562 and Daudi cell lines. Clone 30 expressed alpha beta TCR, and was devoid of cytotoxic activity. Human T cell lines and clones specific to retinal antigens will provide the framework necessary to examine the events that lead to ocular inflammation.

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Selected References

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  1. Abo T., Sugawara S., Seki S., Fujii M., Rikiishi H., Takeda K., Kumagai K. Induction of human TCR gamma delta + and TCR gamma delta-CD2+CD3- double negative lymphocytes by bacterial stimulation. Int Immunol. 1990;2(8):775–785. doi: 10.1093/intimm/2.8.775. [DOI] [PubMed] [Google Scholar]
  2. Caspi R. R., Roberge F. G., McAllister C. G., el-Saied M., Kuwabara T., Gery I., Hanna E., Nussenblatt R. B. T cell lines mediating experimental autoimmune uveoretinitis (EAU) in the rat. J Immunol. 1986 Feb 1;136(3):928–933. [PubMed] [Google Scholar]
  3. Donoso L. A., Yamaki K., Merryman C. F., Shinohara T., Yue S., Sery T. W. Human S-antigen: characterization of uveitopathogenic sites. Curr Eye Res. 1988 Nov;7(11):1077–1085. doi: 10.3109/02713688809001878. [DOI] [PubMed] [Google Scholar]
  4. Dorey C., Cozette J., Faure J. P. A simple and rapid method for isolation of retinal S antigen. Ophthalmic Res. 1982;14(4):249–255. doi: 10.1159/000265199. [DOI] [PubMed] [Google Scholar]
  5. Gregerson D. S., Fling S. P., Merryman C. F., Zhang X. M., Li X. B., Heber-Katz E. Conserved T cell receptor V gene usage by uveitogenic T cells. Clin Immunol Immunopathol. 1991 Jan;58(1):154–161. doi: 10.1016/0090-1229(91)90157-6. [DOI] [PubMed] [Google Scholar]
  6. Gregerson D. S., Merryman C. F., Obritsch W. F., Donoso L. A. Identification of a potent new pathogenic site in human retinal S-antigen which induces experimental autoimmune uveoretinitis in LEW rats. Cell Immunol. 1990 Jun;128(1):209–219. doi: 10.1016/0008-8749(90)90019-n. [DOI] [PubMed] [Google Scholar]
  7. Groh V., Porcelli S., Fabbi M., Lanier L. L., Picker L. J., Anderson T., Warnke R. A., Bhan A. K., Strominger J. L., Brenner M. B. Human lymphocytes bearing T cell receptor gamma/delta are phenotypically diverse and evenly distributed throughout the lymphoid system. J Exp Med. 1989 Apr 1;169(4):1277–1294. doi: 10.1084/jem.169.4.1277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Heber-Katz E., Acha-Orbea H. The V-region disease hypothesis: evidence from autoimmune encephalomyelitis. Immunol Today. 1989 May;10(5):164–169. doi: 10.1016/0167-5699(89)90174-6. [DOI] [PubMed] [Google Scholar]
  9. Hirose S., Kuwabara T., Nussenblatt R. B., Wiggert B., Redmond T. M., Gery I. Uveitis induced in primates by interphotoreceptor retinoid-binding protein. Arch Ophthalmol. 1986 Nov;104(11):1698–1702. doi: 10.1001/archopht.1986.01050230136049. [DOI] [PubMed] [Google Scholar]
  10. Hirose S., McAllister C. G., Mittal K. K., Vistica B. P., Shinohara T., Gery I. A cell line and clones of lymphocytes from a healthy donor, with specificity to S-antigen. Invest Ophthalmol Vis Sci. 1988 Nov;29(11):1636–1641. [PubMed] [Google Scholar]
  11. Matsumoto M., Yasukawa M., Inatsuki A., Kobayashi Y. Human double-negative (CD4-CD8-) T cells bearing alpha beta T cell receptor possess both helper and cytotoxic activities. Clin Exp Immunol. 1991 Sep;85(3):525–530. doi: 10.1111/j.1365-2249.1991.tb05761.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mochizuki M., Kuwabara T., McAllister C., Nussenblatt R. B., Gery I. Adoptive transfer of experimental autoimmune uveoretinitis in rats. Immunopathogenic mechanisms and histologic features. Invest Ophthalmol Vis Sci. 1985 Jan;26(1):1–9. [PubMed] [Google Scholar]
  13. Nussenblatt R. B., Gery I., Ballintine E. J., Wacker W. B. Cellular immune responsiveness of uveitis patients to retinal S-antigen. Am J Ophthalmol. 1980 Feb;89(2):173–179. doi: 10.1016/0002-9394(80)90108-7. [DOI] [PubMed] [Google Scholar]
  14. Nussenblatt R. B., Kuwabara T., de Monasterio F. M., Wacker W. B. S-antigen uveitis in primates. A new model for human disease. Arch Ophthalmol. 1981 Jun;99(6):1090–1092. doi: 10.1001/archopht.1981.03930011090021. [DOI] [PubMed] [Google Scholar]
  15. Nussenblatt R. B., Mittal K. K., Ryan S., Green W. R., Maumenee A. E. Birdshot retinochoroidopathy associated with HLA-A29 antigen and immune responsiveness to retinal S-antigen. Am J Ophthalmol. 1982 Aug;94(2):147–158. doi: 10.1016/0002-9394(82)90069-1. [DOI] [PubMed] [Google Scholar]
  16. Nussenblatt R. B., Palestine A. G., El-Saied M., Meyers S., Lando Z., Mullenberg C., Rozenszajn L. A. Long-term antigen specific and non-specific T-cell lines and clones in uveitis. Curr Eye Res. 1984 Feb;3(2):299–305. doi: 10.3109/02713688408997213. [DOI] [PubMed] [Google Scholar]
  17. Sprent J., Schaefer M. Antigen-presenting cells for unprimed T cells. Immunol Today. 1989 Jan;10(1):17–23. doi: 10.1016/0167-5699(89)90060-1. [DOI] [PubMed] [Google Scholar]
  18. Suzuki Y., Hoshi K., Matsuda T., Mizushima Y. Increased peripheral blood gamma delta+ T cells and natural killer cells in Behçet's disease. J Rheumatol. 1992 Apr;19(4):588–592. [PubMed] [Google Scholar]
  19. Yamaki K., Tsuda M., Shinohara T. The sequence of human retinal S-antigen reveals similarities with alpha-transducin. FEBS Lett. 1988 Jul 4;234(1):39–43. doi: 10.1016/0014-5793(88)81298-5. [DOI] [PubMed] [Google Scholar]
  20. Yamamoto J. H., Minami M., Inaba G., Masuda K., Mochizuki M. Cellular autoimmunity to retinal specific antigens in patients with Behçet's disease. Br J Ophthalmol. 1993 Sep;77(9):584–589. doi: 10.1136/bjo.77.9.584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yamamoto J. H., Okajima O., Mochizuki M., Shinohara T., Wiggert B., Chader G. J., Gery I., Nussenblatt R. B. Cellular immune responses to retinal antigens in retinitis pigmentosa. Graefes Arch Clin Exp Ophthalmol. 1992;230(2):119–123. doi: 10.1007/BF00164648. [DOI] [PubMed] [Google Scholar]
  22. Yang S. Y., Rhee S., Welte K., Dupont B. Differential in vitro activation of CD8-CD4+ and CD4-CD8+ T lymphocytes by combinations of anti-CD2 and anti-CD3 antibodies. J Immunol. 1988 Apr 1;140(7):2115–2120. [PubMed] [Google Scholar]

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