Fig. 1.

Human RORα4 transgene tissue specificity, growth curve and tissue weights of WT versus Tg-FABP4-RORα4 mice on normal chow. (A–C) RNA was extracted from selected tissues (kidney, quadriceps muscle, liver, SAT (inguinal fat), visceral adipose (epididymal fat) and brown adipose (interscapular fat), and examined for relative gene expression of (A) endogenous (mouse) Rorα1 and Rorα4, (B) the transgene (human RORα4) and (C) total RORα (mouse and human) compared to the endogenous control (18s) using real-time PCR (n = 3 littermate pairs in duplicate experiments). Statistical analyses were performed using unpaired two-tailed Student's t-test for (A) and two-way ANOVAs with Bonferroni's post-test applied for (B–C) where *P < 0.05; **P < 0.01; ***P < 0.001. (D) Schematic timeline of diet regime employed. (E) Body weight (in grams) of male WT (black) and tg/+ (red) mice (n = 18 littermate pairs) measured weekly up to 14 weeks of age. Statistical analyses were performed using two-way ANOVAs with Bonferroni's post-test applied but results were not significant. (F) Representative images of dissected WT and tg/+ littermate pair (chow diet). (G) Mean relative mass/total weight ± S.E.M. of adipose tissues dissected from WT and tg/+ mice (n = 7 littermate pairs). Statistical analyses were performed on each tissue using unpaired two-tailed Student's t-test was performed where *P < 0.05.